Project description:The whole-genome oligonucleotide microarray analysis of NS398-treated HT29 colon adenocarcinoma cells samples can give an insight into global molecular background of selective COX2 inhibitor administration in order to find other target molecules and pathways influenced by NS398 selective COX2 inhibitor treatment in the epithelial cells. Total RNA was extracted from NS398-treated and control HT29 colon adenocarcinoma cells and hybridized on Affymetrix HGU133 Plus 2.0 microarrays
Project description:human colon cancer cell line HT29 cells treated with 0.5μM thapsigargin 2.5hours. Compared with equal amount total RNA from nontreated control cells. qPCR gene expression profiling.
Project description:The whole-genome oligonucleotide microarray analysis of NS398-treated HT29 colon adenocarcinoma cells samples can give an insight into global molecular background of selective COX2 inhibitor administration in order to find other target molecules and pathways influenced by NS398 selective COX2 inhibitor treatment in the epithelial cells.
Project description:human colon cancer cell line HT29 cells treated with 0.5μM thapsigargin 2.5hours. Compared with equal amount total RNA from nontreated control cells. qPCR gene expression profiling. one sample