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ABSTRACT: metatranscription sequencing of mycoviruses in Rhizoctonia solani

PROVIDER: PRJNA1170489 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30970536_1.fastq.gz	Fastqsanger.gz
	SRR30970536_2.fastq.gz	Fastqsanger.gz

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Exploring pathogenic microRNAs of rice sheath blight pathogen

Project description:We constructed seven small RNA libraries of Rhizoctonia solani AG1 IA and sequenced using Illumina GA II. The seven samples include mycelium cultured on PDA without rice incubated, 6 different stages at 10 hours (10h), 18h, 24h, 32h, 48h and 72h spanning the Rhizoctonia solani AG1 strains infection rice. We identified miRNA-like small RNAs (milRNAs) using MIREAP and mirdeep2. The milRNAs were used for further analysis of interactions between milRNA and mRNA that may involve in plant-infection.

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Project description:Rhizoctonia solani is an economically important soil-borne necrotrophic fungal pathogen, with a broad host range and for which little effective resistance exists in crop plants. Arabidopsis is resistant to the R. solani AG8 isolate but susceptible to R. solani AG2-1. Affymetrix microarray analysis was performed to determine genes that are affected in common and specifically by AG8 and AG2-1.

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