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High Expression of SERPINE1 and CTSL in Keratinocytes in Pressure Injury Caused by Ischemia-reperfusion Injury

ABSTRACT: High Expression of SERPINE1 and CTSL in Keratinocytes in Pressure Injury Caused by Ischemia-reperfusion Injury

PROVIDER: PRJNA1171330 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30942184_1.fastq.gz	Fastqsanger.gz
	SRR30942184_2.fastq.gz	Fastqsanger.gz
	SRR30942185_1.fastq.gz	Fastqsanger.gz
	SRR30942185_2.fastq.gz	Fastqsanger.gz
	SRR30942186_1.fastq.gz	Fastqsanger.gz

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High Expression of SERPINE1 and CTSL in Keratinocytes in Pressure Injury Caused by Ischemia-reperfusion Injury

Project description:Pressure Injury (PI) is a complex disease process which is influenced by multiple factors, among which ischemia-reperfusion (I/R) injury is closely related to the progression of PI. But, its biomarkers are still unclearly. Understanding its physiological mechanisms and related molecular biomarkers is the key to developing effective prevention and therapeutic strategies. This study through obtained the candidate genes of the differentially expressed genes (DEGs) from the PI rat model by transcriptome sequencing, PI single-cell sequencing database, and genes related to ischemic-reperfusion (I/R) injury from GeneCards database to analyze and screen prognostic related target genes. There were finally 7 candidate genes obtained from the intersection of the three datasets. Gene Ontology (GO) and Kyoto Encyclopedia of Genomes (KEGG) pathway analysis revealed that the DEGs were enriched in complement and coagulation cascades, and the keratinocyte differentiation is a significant factor. Then, two hub genes Serine protease inhibitor clade E member 1 (SERPINE1) and Cathepsin L (CTSL) were identified through three machine learning algorithms. The two hub genes were assessed using the nomogram and Receiver Operating Characteristic (ROC) approaches, and the findings indicated that these two genes had a high prognosis value. The two genes both play crucial roles in vasculogenesis, coagulation and degradation of the extracellular matrix, which is essential for wound healing. The results of immunohistochemistry demonstrated that SERPINE1 and CTSL are significantly upregulated in skin tissue from PI caused by I/R injury, and their mRNA expression levels significantly correlate with PI outcomes. According to our research we referred that the SERPINE1 and CTSL might be the potential biomarkers of PI.

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