Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Staphylococcus aureus bloodstream infection mouse metagenome

PROVIDER: PRJNA1171428 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30946000_1.fastq.gz	Fastqsanger.gz
	SRR30946000_2.fastq.gz	Fastqsanger.gz
	SRR30946001_1.fastq.gz	Fastqsanger.gz
	SRR30946001_2.fastq.gz	Fastqsanger.gz
	SRR30946002_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Identification of a novel LysR-type transcriptional regulator in Staphylococcus aureus that is crucial for secondary tissue colonization during metastatic bloodstream infection

Project description:Purpose: The goal of this study was to identify Staphylococcus aureus genes which are important during establishment of metastatic infections in bloodstream infections. Methods: Pooled mariner transposon mutant libraries were generated as previously described (PMID: 27185949). The libraries were sequenced on the Illumina HiSeq 2500 platform with the transposon-specific oligonucleotide primer Himar1-Seq. Illumina adapter sequences were removed via cutadapt version 1.2.1. The reads were filtered for size (>16 bp) and to contain the signature transposon inverted repeat. Reads were mapped to the Staphylococcus aureus 6850 genome (RefSeq accession NC_022222.1) by Bowtie2 v2.1.0. Identification of depleted and enriched mutants was performed via DESeq2 version 1.6.2. Results: S. aureus genes were identified which were upregulated or downregulated upon iduced expression of the LysR-type transcriptional regulator RSAU_000852 in Staphylococcus aureus 6850. Conclusions: Our study identifies a gene for a LysR-type transcription regulator, which is involved in metabolic adaptation of the pathogen during colonization of secondary infection sites in a bloodstream infection model.

2020-09-08 | GSE139513 | GEO

Transcription profiling of human HUVEC cells with internalised S. aureus

Project description:Staphylococcus aureus is a Gram-positive bacteria frequently isolated from patients with bloodstream infections. Endothelial cells (ECs) play an important role in host cell defence against bacteria, and recent reports have shown that infection of ECs with S. aureus induces a wide range of cytokines and cell surface receptors involved in activating the innate immune response (). The ability of S. aureus to invade nonphagocytic cells, including ECs, has been documented (), however, the knowledge of ECs role in pathogenesis of S. aureus infection is still limited. <br> In this study, we investigate the gene expression program initiated in human ECs by internalized S. aureus, using microarray analysis. We found 156 genes differentially regulated at least threefold, using arrays representing 14 239 genes. The main part of the upregulated genes code for cytokines, cell adhesion molecules, molecules involved in antigen presentation, cell signaling or cell metabolism. A variety of cytokines and chemokines seem to play an important role in S. aureus infection. Despite an apparent inflammatory response, internalized bacteria survived without inducing EC death.<br>

2005-10-31 | E-MEXP-226 | biostudies-arrayexpress

Staphylococcus aureus induces a muted host response in human blood that blunts the recruitment of neutrophils

Project description:Methicillin resistant Staphylococcus aureus (MRSA) is an opportunistic pathogen chief amongst bloodstream infecting pathogens. MRSA produces an array of human specific virulence factors that may contribute to immune suppression. Here, we defined the response of primary human phagocytes to infection with MRSA using RNA-Seq. We found that the overall transcriptional response to MRSA was weak both in the number of genes and the magnitude of response. Using an ex vivo bacteremia model with fresh human blood, we found that infection with live MRSA resulted in the down-regulation of genes related to innate immune response, and cytokine and chemokine signaling. This muted transcriptional response was conserved across diverse S. aureus clones but absent in heat-killed MRSA or blood infected with live Staphylococcus epidermidis. Importantly, the muted signature was also present in patients with S. aureus bacteremia. We next identified the master regulator SaeRS and the SaeRS-regulated pore-forming toxins as key mediators of transcriptional suppression. The impaired chemokine and cytokine responses were reflected by circulating protein levels in the plasma. MRSA elicits a soluble milieu that is restrictive in the recruitment of human neutrophils compared to strains lacking saeRS. Thus, MRSA blunts the inflammatory response resulting in impaired neutrophil recruitment, which could promote the survival of S. aureus during invasive infection.

2022-07-20 | GSE193219 | GEO

Caenorhabditis elegans infected with Staphylococcus aureus

Project description:Young adult fer-15;fem-1 Caenorhabditis elegans were infected with Staphylococcus aureus for 8 h to determine the transcriptional host response to Staphylococcus aureus. Analysis of differential gene expression in C. elegans young adults exposed to two different bacteria: E. coli strain OP50 (control), wild-type Staphylococcus aureus RN6390. Samples were analyzed at 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Keywords: response to pathogen infection, innate immunity, host-pathogen interactions

2010-06-12 | GSE21819 | GEO

Gene expression profiles of ears infected with Staphylococcus aureus at ZT4

Project description:We analysed the gene expression profiles after infection of Staphylococcus aureus at ZT4 in CXCL14-KO mice. The group of genes categolized as GO defense response (GO: 006952) were upregulated upon a response to Staphylococcus aureus infection. The expression of IL1b, Cxcl2, and Ccl3 were significantly suppressed in infected ear of Cxcl14-KO compared to that of WT.

2022-03-31 | GSE164899 | GEO

Wound Coinfection- Run 2 Sept 4

Project description:in vivo mouse study of a pseudomonas aeruginosa and staphylococcus aureus infection monoinfection and coinfection wound. tissue samples separated by region for untargeted metabolomics study

| MSV000090290 | MassIVE

Single cells RNA-Seq of CD45+ cells during Staphylococcus aureus craniotomy infection

Project description:Single cells RNA-Seq of CD45+ cells during Staphylococcus aureus craniotomy infection

2020-12-07 | GSE153435 | GEO

Full genome qRT PCR of Staphylococcus aureus strains USA300 and TB15 during infection of 5 organs

Project description:We used high-throughput qRT-PCR analysis to obtain full genome qRT- PCR data for the Staphylococcus aureus strains USA300 (wild type) and TB15 (mutant). Both strains were collected during infection from 4 mouse organs ( skin, kidney, lung,liver) as well as from human neutrophil infection.

2011-06-13 | GSE24701 | GEO

IκBζ-dependent responses in keratinocytes upon Staphylococcus aureus infection

Project description:In this study we investigated the transcriptional responses of keratinocyte-derived IκBζ (encoded by NFKBIZ) upon infection with the pathogenic Staphylococcus aureus (S. aureus) USA300 wildtype strain.

2025-04-23 | GSE267665 | GEO

Neutrophil-derived IL-1β is sufficient for abscess formation in immunity against Staphylococcus aureus in mice

Project description:Neutrophil abscess formation is critical in innate immunity against many pathogens. Here, the mechanism of neutrophil abscess formation was investigated using a mouse model of Staphylococcus aureus cutaneous infection. Gene expression analysis of S. aureus-infected skin revealed that induction of neutrophil recruitment genes was largely dependent upon IL-1beta/IL-1R activation. Unexpectedly, using IL 1beta reporter mice, neutrophils were identified as the primary source of IL-1beta at the site of infection. Furthermore, IL-1beta-producing neutrophils were necessary and sufficient for abscess formation and bacterial clearance. S. aureus-induced IL 1beta production by neutrophils required TLR2, NOD2, FPRs and the ASC/NLRP3 inflammasome. Taken together, IL-1beta and neutrophil abscess formation during an infection are functionally, spatially and temporally linked as a consequence of direct IL-1beta production by neutrophils. Lesional skin biopsies obtained from C57BL/6J WT mice or IL-1R-deficient mice at 4 hours post-infection with Staphylococcus aureus. Uninfected skin biopsies were also collected from WT and IL-1R-deficient mice.

2012-12-05 | E-GEOD-36826 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data