Effect of IFNT and PGF2A on gene expression of cultured luteal steroidogenic cells [Cultured cells]
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1172689 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR30985668_1.fastq.gz | Fastqsanger.gz | |||
| SRR30985668_2.fastq.gz | Fastqsanger.gz | |||
| SRR30985669_1.fastq.gz | Fastqsanger.gz | |||
| SRR30985669_2.fastq.gz | Fastqsanger.gz | |||
| SRR30985670_1.fastq.gz | Fastqsanger.gz |
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Project description:The effect of interferon tau (IFNT) on the uterus is critical for maternal recognition of pregnancy in ruminants, while its direct role in luteal function is less well understood. To address this, we performed two experiments. In experiment 1, cattle received intrauterine infusions of one of four treatments: bovine serum albumin (BSA; vehicle) from day 14 to 16 of the estrous cycle, vehicle with IFNT from day 14 to 16 (3-day IFNT), vehicle with IFNT from day 14 to 19 (6-day IFNT), and vehicle with IFNT from day 14 to 16 followed by vehicle with IFNT and pregnancy associated glycoprotein (PAG) from day 17 to 19 (IFNT+PAG). Corpora lutea (CL) were collected on day 17 or 20 and RNAseq was performed. In experiment 2, cultured luteal steroidogenic cells from cyclic (day 10-12) cattle were treated with IFNT and RNAseq was performed. In both experiments, IFNT resulted in luteal changes (in vivo: 130 transcripts; in vitro: 2981 transcripts), while additional treatment with PAG resulted in only 13 changed transcripts. Only 31% of the genes that changed in the CL during early pregnancy (Hughes et al., 2020) were regulated by IFNT. Gene ontology analysis revealed that these genes were regulators of antiviral and immune responses. In contrast, 50% of the genes that changed during early pregnancy were not regulated by IFNT in vivo or in vitro and were associated with cellular proliferation and extracellular matrix organization. It is suggested that the temporal changes in the CL during early pregnancy are only partially regulated directly by IFNT, drawing into question identities of other luteal regulators or the effect of age of CL.
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Project description:The effect of interferon tau (IFNT) on the uterus is critical for maternal recognition of pregnancy in ruminants, while its direct role in luteal function is less well understood. To address this, we performed two experiments. In experiment 1, cattle received intrauterine infusions of one of four treatments: bovine serum albumin (BSA; vehicle) from day 14 to 16 of the estrous cycle, vehicle with IFNT from day 14 to 16 (3-day IFNT), vehicle with IFNT from day 14 to 19 (6-day IFNT), and vehicle with IFNT from day 14 to 16 followed by vehicle with IFNT and pregnancy associated glycoprotein (PAG) from day 17 to 19 (IFNT+PAG). Corpora lutea (CL) were collected on day 17 or 20 and RNAseq was performed. In experiment 2, cultured luteal steroidogenic cells from cyclic (day 10-12) cattle were treated with IFNT and RNAseq was performed. In both experiments, IFNT resulted in luteal changes (in vivo: 130 transcripts; in vitro: 2981 transcripts), while additional treatment with PAG resulted in only 13 changed transcripts. Only 31% of the genes that changed in the CL during early pregnancy (Hughes et al., 2020) were regulated by IFNT. Gene ontology analysis revealed that these genes were regulators of antiviral and immune responses. In contrast, 50% of the genes that changed during early pregnancy were not regulated by IFNT in vivo or in vitro and were associated with cellular proliferation and extracellular matrix organization. It is suggested that the temporal changes in the CL during early pregnancy are only partially regulated directly by IFNT, drawing into question identities of other luteal regulators or the effect of age of CL.
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Project description:Comparative genome wide gene expression profiles of small and large luteal cells from characterized mature CL are not currently available in any species. During present study, transcriptome differences of small and large luteal cells werte comprehensively analyzed to understand the specific functional roles of small and large luteal cells in mature bovine CL.
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