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Project description:Red clover (Trifolium pratense L.) is one of the most important legume forage species in temperate livestock agriculture. Tetraploid red clover cultivars are generally producing less seed than diploid cultivars. Improving the seed setting potential of tetraploid cultivars is necessary in order to utilize the high forage quality and environmentally sustainable nitrogen fixation of red clover. Two genotypes, one from cv.Tripo with weak seed setting and one from cv.Lasang with strong seed setting, were selected based on data from field experiments for transcriptome analysis of developing flower buds. De novo and reference based analyses of transcriptome assemblies were conducted to study the global transcriptome changes from early to late developmental stages of flower development of the two contrasting red clover genotypes. Transcript profiles, gene ontology enrichment and KEGG pathway analysis indicate that genes related to flower development, pollen pistil interactions, photosynthesis and embryo development are differentially expressed between the Tripo and Lasang genotypes. A significant number of genes related to pollination was overrepresented in Lasang, which might be a reason for its good seed setting ability. The candidate genes detected in this study might be used to develop molecular tools for breeding tetraploid red clover varieties with improved seed yield potentials.
2017-01-31 | E-MTAB-5117 | biostudies-arrayexpress
Project description:Helminth infections, such as gastrointestinal nematodes (GIN) are highly prevalent in grazing-based livestock systems, compromising animal health and welfare and causing substantial economic losses. Although anthelmintics remain the primary control strategy, resistance is becoming increasingly widespread. Thus, sustainable alternatives are urgently needed to improve parasite control and support animal health. We formulated a red clover–based forage diet using the biorefinery by-product red clover pulp. In sheep infected with three common GIN species, this diet reduced fecal egg counts by ~40% (P = 0.0006) without negatively affecting feed intake or weight gain. The diet also significantly increased the abundance of Bacteroidales RF16 and Bacteroidales p-251-o5, taxa associated with short-chain fatty acid production, while reducing Methanomethylophilaceae, a family linked to methane production. Moreover, the red clover diet markedly altered the abomasal environment, and these changes were mirrored by shifts in the Haemonchus contortus gut microbiome, whereas the microbiomes of Teladorsagia circumcincta and Trichostrongylus colubriformis remained largely unaffected by diet. These findings highlight a sustainable dietary strategy to reduce GIN transmission and improve ruminant health, while also revealing distinct interactions between host and parasite-associated microbiomes that could be key to designing future control strategies.
2026-06-01 | GSE325079 | GEO
Project description:Traditional medicinal plants contain a variety of bioactive natural products including cysteine-rich (Cys-rich) antimicrobial peptides (AMPs). Cys-rich AMPs are often crosslinked by multiple disulfide bonds which increase their resistance to chemical and enzymatic degradation. However, this class of molecules is relatively underexplored. Herein, in silico analysis predicted 80 – 100 Cys-rich AMPs per species from three edible traditional medicinal plants: Linum usitatissimum (flax), Trifolium pratense (red clover), and Sesamum indicum (sesame). Proteomics analysis of seed peptide extracts revealed direct evidence for the translation of 3-10 Cys-rich AMPs per species including lipid transfer proteins, defensins, α-hairpinins, and snakins. Negative activity revealed by antibacterial screening highlights the importance of employing a multi-pronged approach for AMP discovery. Further, this study demonstrates that flax, red clover, and sesame are promising sources of further AMP discovery and characterization.
2022-02-16 | PXD029289 | Pride