SSEA-1+ and SSEA-1- endometrial epithelial cells
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1177846 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:SSEA-1+ endometrial epithelial cells (EECs) assume the postulated stem progenitor cell niche within the human endometrium. Previous studies have demonstrated isolated SSEA-1+ cells to have a higher capacity to generate organoids in a 3D matrix, have lower steroid hormone expression and higher telomerase activity with longer telomere lengths. To further explore this we conducted transcriptional profiling comparing human SSEA-1 enriched and depleted endometrial epithelial cells from eight individuals.
2025-05-07 | GSE280323 | GEO
Project description:Synchronized cross talk between embryo and endometrium during pre-implantation period is critical for establishment of pregnancy. Extracellular vesicels(EVs) of both embryo and endometrial origin are known to be integral in regulating embryo maternal communication during this time. However, exact molecular signalling pathways or factors that regulate the embryo endometrial communication during pre-conception period remains elusive. Here in this study extracellular vesicles from trophoblast cells were shown to modulate endometrial epithelial cell secretome in favour of embryo implantation and embryo development.
2023-10-24 | PXD040311 | Pride
Project description:microRNAs are essential regulators of gene expression. This work explores the transcriptomic effects of has-miR-30d in endometrial epithelial cells which was reported to be up-regulated during human endometrial receptivity. primary endometrial epithelial cells derived from four different endometrial biopsies taken on LH+0 were transiently transfected with miRNAs for 72 hours under two conditions (four scramble, four miR-30d mimic) and were evaluated for mRNA expression with Agilent’s gene expression microarray. A set of differentially expressed mRNAs were validated by qPCR.
2014-02-03 | E-GEOD-46721 | biostudies-arrayexpress
Project description:The role of obesity in endometrial cancer development is tested by co-culturing adipose stromal cells (ASCs) with endometrial epithelial cells and endometrial cancer cell Ishikawa for 21 days. Control cells (not exposed to ASCs) were incubated for the same duration. RNA-seq identified differential expression due to ASC exposure
2019-02-13 | GSE103203 | GEO
Project description:Extensive trauma disrupts endometrial regeneration by diminishing endometrial stem or progenitor cells. Endometrial epithelial organoids (EEOs) are used for endometrial regeneration. However, how EEOs repair injured endometrial tissues and their advantages over bone marrow mesenchymal stem cells (BMSCs) are unclear. This study explored whether EEOs surpass BMSCs to repair injured endometrium and examined whether endometrial restoration involves integrating EEOs into the endometrial tissue of the recipient. Rat EEOs (rEEOs) mimicking the features of the rat endometrium were developed. An endometrial injury rat model was used to compare the effects of rEEOs and rat BMSCs (rBMSCs) on endometrial regeneration and reproductive recovery. Bulk RNA-sequencing analysis was conducted to investigate the capacity of rEEOs for endometrial regeneration and identify discrepancies between rEEOs and rBMSCs. Green fluorescent protein (GFP)-labeled rEEOs or red fluorescent protein-labeled rBMSCs were transplanted to track the transplanted cells in vivo. Fertility recovery in rats transplanted with rEEOs was more comparable to that of normal rats than in rBMSC-treated rats. rEEOs had a high concentration of endometrial epithelial stem or progenitor cells and secreted vascular endothelial growth factor-A to promote endometrial neovascularization. Cells from GFP-labeled rEEOs integrated and differentiated into functional glands within the injured endometrium. Transplanting EEOs restored the morphology and function of severe endometrial injury better than BMSC transplantation. EEO cells repair the endometrium by differentiating into functional glandular epithelial cells. These findings offer preclinical evidence supporting in vitro expansion and transplanting of EEOs as promising approaches to restore fertility in women with insufficient endometrial regeneration.
2024-11-29 | GSE269747 | GEO
Project description:microRNAs are essential regulators of gene expression. This work explores the transcriptomic effects of has-miR-30d in endometrial epithelial cells which was reported to be up-regulated during human endometrial receptivity.
2014-02-03 | GSE46721 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE29435: Progesterone inhibits epithelial-to-mesenchymal transition in endometrial cancer: cell line data GSE29436: Progesterone inhibits epithelial-to-mesenchymal transition in endometrial cancer: patient data Refer to individual Series
2012-01-26 | E-GEOD-29437 | biostudies-arrayexpress
Project description:96 CD326+ single cells from post-natal day7 mice endometrial epithelial cells were isolated by FACS single cell pre-amplification and Q-PCR were then conducted
2017-06-30 | GSE98438 | GEO
Project description:We aimed to improve the efficiency of isolating endometrial epithelial and stromal cells (EMECs and EMSCs) from hysterectomy specimens. We revealed by immunohistochemical staining that the large tissue fragments remaining after collagenase treatment, which are usually discarded after the first filtration in the conventional protocol, consisted of glandular epithelial and stromal cells. Therefore, we established protease-treatment and cell suspension conditions to dissociate single cells from the tissue fragments, and isolated epithelial (EPCAM-positive) and stromal (CD13-positive) cells by fluorescence-activated cell sorting. Four independent experiments showed that, on average, 1.2 x 10^6 of EMECs and 2.8 x 10^6 EMSCs were isolated from one hysterectomy specimen. We confirmed that the isolated cells presented transcriptomic features highly similar to those of epithelial and stromal cells obtained by the conventional method. Our improved protocol facilitates future studies to better understand the epigenetic regulation underlying the dynamic changes of the endometrium during the menstrual cycle. Although protocols for the isolation of endometrial stromal and epithelial cells (EMSCs and EMECs) have been well established, the number of EMECs obtainable by the current protocols is relatively small. To improve the efficiency of isolating EMECs as well as EMSCs from endometrial tissues, we established protease-treatment and cell suspension conditions to efficiently dissociate single cells from endometrial tissue fragments, and isolated epithelial (EPCAM-positive) and stromal (CD13-positive) cells by fluorescence-activated cell sorting. By conducting a microarray-based transcriptome analysis, we confirmed that the cells isolated by the modified protocol developed in this study maintain the transcriptomic properties of endometrial epithelial and stromal cells.
2016-06-14 | E-GEOD-73090 | biostudies-arrayexpress
Project description:The human endometrium is a highly regenerative tissue that undergoes cyclical proliferation, differentiation and shedding each month. The upper functionalis layer of the endometrium is shed in response to circulating levels of estrogen and progesterone, while the lower basalis layer remains. Clonogenic epithelial stem/progenitor cells likely responsible for regenerating endometrial epithelium have been identified in pre-menopausal (Pre-M) and post-menopausal (Post-M) endometrium and may reside in the basalis layer. We undertook a transcriptional profiling of purified epithelial cells from full-thickness Pre-M and Post-M endometrium to identify differentially expressed genes. The hypothesis tested in the present study was that Post-M endometrial epithelial gene profile would be similar to the quiescent basalis epithelium of Pre-M endometrium. We found striking differential gene expression of many Wnt family members between Pre-M and Post-M and other stem cell network genes. Comparative analysis of our endometrial epithelial gene expression profiles to that of endometrial epithelial cells in remodelling endometrium also provides new evidence showing that Post-M endometrial epithelium has a similar gene signature to that of basalis epithelium of menstrual endometrium. Human endometrial tissue was obtained from 8 pre-menopausal and 3 post-menopausal women undergoing hysterectomy for various benign gynaecologic conditions. Endometrial tissue was digested and isolated using combination of DNase and collagenase. Anti-human EpCAM antibody-coated magnetic Dynabeads was used to positively select total epithelial cells from the digested single cell suspensions. Total RNA was extracted from the purified endometrial epithelial cells and hybridised to Illumina Sentrix HT12 beadchip. Resulting data was compared between pre-menopausal and post-menopausal samples.
2012-04-10 | E-GEOD-35221 | biostudies-arrayexpress