Project description:Proteomic analysis was performed on the proximal tibia head from healhty and BCO-affected broiler (meat-type) chickens using Bruker Daltonics amaZon series Mass Spectrometer. This analysis was undertaken to identify key protein signature involved in BCO, a common cause of lameness.
Project description:DNA was isolated from whole red blood cells from various lines and crosses of broiler chickens. DNA was genotyped using Axiom genome-wide chicken array and cel files were analyzed using Axiom Analysis Suite Software (version 3.0.1) with Gallus gallus 5.0 using the software's Best Practices for agricultural animals. The results were exported (Genotyping_Data-3-21-2018.vcf) for all genotype calls and text file of all SNPs with >= 97% call rate rate was also produced for filtering the VCF file (ALL_SNPSs_with_Call_Rate_97_Plus_3-21-2018).
Project description:This study investigates the impact of stress on muscle physiology and meat quality in broiler chickens by comparing protein expression profiles between organic and conventional farming systems using label-free quantitative (LFQ) proteomics. Muscle samples were analyzed via nanoLC-ESI-MS/MS coupled with comprehensive bioinformatics to identify differences in protein abundance associated with rearing conditions.A total of 7,221 proteins were identified, with 1,645 proteins upregulated and 1,612 downregulated in organic chickens compared to conventional ones. Functional analyses including Gene Ontology (GO) and STRING network analyses revealed that proteins upregulated in organic chickens were predominantly involved in oxygen transport, oxygen binding, and muscle structural organization, indicating enhanced oxygen metabolism and muscle development consistent with improved animal welfare. Conversely, proteins related to ribosomal function and RNA binding were enriched in conventional chickens, suggesting stress-related alterations in protein synthesis. KEGG pathway analysis showed significant enrichment of carbon metabolism, amino acid biosynthesis, nitrogen metabolism, and the tricarboxylic acid (TCA) cycle pathways in organic chickens, while glycolysis, gluconeogenesis, and ribosomal pathways were downregulated. Key differentially expressed proteins identified as potential biomarkers distinguishing organic from conventional meat include downregulated PGM1, AMPD1, LDHA, ENO3, and PKLR, and upregulated COL1A1, COL1A2, TTN, TPM2, CA3, MB, HSPB1, ACO2, ACAA2, and TF. These proteins are involved in muscle structure and energy metabolism and may serve as indicators of meat quality linked to stress and welfare conditions. Overall, this proteomic analysis provides novel insights into how stress modulates the muscle proteome in broiler chickens and supports the adoption of welfare-focused organic poultry production practices to improve meat quality.
Project description:Gene expression profiling of male broiler chickens exposed to APEC O1. Comparisons were made between Day 1 and Day 5 of all treatment groups, between differences in pathology and effect of vaccine on spleen gene expression. The goal was to determine expression differences that could convey genetic resistance to APEC O1.
Project description:The process of commercial catching, transport and slaughter (CTS) is known to be an acute stressful event in broiler chickens. Corticosteroid concentrations increase, impacting measures of IGF-1, growth hormone and metabolites of the immune system from blood plasma samples. We used ARK-Genomics chicken 20K oligo array, a two channel DNA microarray, to investigate the significantly differentially expressed genes in the livers of chickens during CTS.