Thymosin α1 Inhibiting Ferroptosis of Dental Pulp Cells in Pulpitis
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PROVIDER: PRJNA1179354 | ENA |
REPOSITORIES: ENA
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Project description:Tooth pulpitis is a common oral ailment. Understanding the underlying mechanisms of pulpitis and developing effective treatment strategies are of significant importance.In this study, single-cell RNA sequencing (scRNA-seq) was used to identify cellular heterogeneity between 3 pulpitis tissue and 3 healthy pulp tissue. Raw data of ScRNA-seq of 3 healthy pulp tissue have been uploaded (GEO accession: GSE274562; Control1: GSM8452929; Control2: GSM8452930; Control3: GSM8452931).
2025-05-01 | GSE280528 | GEO
Project description:The study investigates the effects of exosomes derived from human dental pulp stem cells (hDPC-Exos) on pulpitis, an inflammatory dental condition. The research demonstrates that hDPC-Exos exhibit significant anti-inflammatory and regenerative properties, making them effective biomaterials for treating pulpitis. Exosomes were shown to downregulate pro-inflammatory cytokines and chemokines, such as interleukin-1β and tumor necrosis factor-alpha, thus suppressing inflammation. Additionally, hDPC-Exos promoted angiogenesis and odontogenesis, facilitating recovery and regeneration of pulp tissue. These effects were observed through various analyses, including RNA sequencing and histological evaluations in an in vitro pulpitis model and a rat model of pulpitis. The findings suggest that hDPC-Exos hold potential as a cell-free therapeutic approach for oral inflammation and tissue regeneration.
2025-04-26 | GSE295186 | GEO
Project description:Purpose: To compare the transcriptional changes of genes in dental pulp tissues with different degrees of inflammatory severity and investigate the role of RAD54B in inflamed human dental pulp cells (hDPCs) Methods: Normal, carious, and pulpitis human dental pulp tissues were collected. Total RNA extracted were subjected to RNA-sequencing and gene expression profiles were further studied by Gene Ontology (GO) and KEGG pathway analysis. DEGs (differentially expressed genes) in homologous recombination repair (HRR) were validated with qRT-PCR. The expression of RAD54B and TNF-α in human dental pulp tissues was detected by immunohistochemistry. HDPCs were cultured and RAD54B level in hDPCs was detected after LPS stimulation using western blot. CCK-8 was applied to investigate the cell proliferation of hDPCs transfected with negative control (Nc) small interfering RNA (siRNA), RAD54B siRNA, P53 siRNA or both siRNAs with or without LPS stimulation. Flow cytometry was applied to detect the cell cycle distribution, and western blot and immunofluorescence were utilized to analyze the expression of RAD54B, P53 and P21 under the above treatments. One-way and two-way ANOVA followed by LSD posttest were used for statistical analysis. Results: RNA-sequencing results identified DEGs among three groups. KEGG pathway analysis revealed enrichment of DEGs in Replication and Repair pathway. HRR and non-homologous end joining (NHEJ) components were further verified and qRT-PCR results were basically consistent with the sequencing data. RAD54B, a HRR accessory factor highly expressed in carious and pulpitis tissues compared to normal pulp, was chosen as our gene of interest. High RAD54B expression was confirmed in inflamed human dental pulp tissues and LPS-stimulated hDPCs. Upon RAD54B knockdown, P53 and P21 expressions in hDPCs were upregulated whereas the cell proliferation was significantly downregulated, accompanied with increased G2/M phase arrest. After inhibiting P53 expression in RAD54B-knockdown hDPCs, P21 expression and cell proliferation were reversed. Conclusions: Gene expression profiles of normal, carious and pulpitis human dental pulp tissues were revealed. HRR components was elucidated to function in dental pulp inflammation. Among HRR DEGs, RAD54B could regulate the cell proliferation of inflamed hDPCs via P53/P21 signaling. This research not only deepens our understanding of dental pulp inflammation but also provides a new insight to clarify the underlying mechanisms.
2023-09-02 | GSE198359 | GEO
Project description:Transcriptional response of rat dental pulp cells (DPCs) cultured with SAHA at early and late mineralisation time points Transcript profiling of DPC identified several novel genes expression induced and supressed by HDACi at 24 hrs and 14 days under mineralising conditions. SAHA induces several members of the MMP family of endopepsidases (TIMP-1, MMP-9, MMP-13) and other members of the endochondral ossification pathway at 24 h. 8 experiemental parameters were analysed, each carried out in quadruplicate
2015-06-16 | E-GEOD-67175 | biostudies-arrayexpress
Project description:Transcriptional response of rat dental pulp cells (DPCs) cultured with SAHA at early and late mineralisation time points Transcript profiling of DPC identified several novel genes expression induced and supressed by HDACi at 24 hrs and 14 days under mineralising conditions. SAHA induces several members of the MMP family of endopepsidases (TIMP-1, MMP-9, MMP-13) and other members of the endochondral ossification pathway at 24 h.
2015-06-16 | GSE67175 | GEO
Project description:Dental pulp inflammation provides an excellent model for the interaction between tissue inflammation and regenerative processes. It is worthwhile to better understand molecular signaling of repair and regeneration in inflammatory processes. Emerging evidence suggests that lncRNAs participate in immune/inflammatory processes. We aimed to investigate the expression of lncRNAs in pulpitis and predict lncRNAs that possibly involved in inflammation and odontogenesis.
2017-12-01 | GSE92681 | GEO
Project description:Gene expression analysis data of human permanent dental pulp tissues
2016-11-22 | GSE75644 | GEO
Project description:Human deciduous and permanent teeth exhibit different developmental processes, morphologies, histological characteristics and life cycles. In addition their pulp tissues react differently to external stimuli, such as the pulp sensitivity test, dental trauma and pulp therapy materials. These differences are attributable to their genetic backgrounds. Therefore the purpose of this study is to compare the differences of dental pulp in deciduous and permanent teeth. Pulp samples were obtained from permanent premolars (n=6, aged 11-14 years) and deciduous teeth (n=6, aged 11-14 years). Comparative cDNA microarrary analysis revealed several differences in gene expression between the deciduous and permanent pulp tissues. Each GSM record represents a pulp sample pooled from two teeth samples.
2014-08-21 | E-GEOD-51449 | biostudies-arrayexpress
Project description:We have performed gene expression microarray analysis to profile transcriptomic signatures affected by EtOH in human dental pulp stem cells Established human dental pulp stem cells were treated with different dose of EtOH (0, 1, 5, 10, 20 and 50mM) for a different time periods (24 and 48 hrs). Total RNA was extracted and subjected to gene expression microarray analysis using Affymetrix human genome 2.0 plus array
2014-05-03 | E-GEOD-57255 | biostudies-arrayexpress