Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile at single cell level of human cortical brain organoids at day 20.

ABSTRACT: Gene expression profile at single cell level of human cortical brain organoids at day 20.

PROVIDER: PRJNA1180996 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR31192920_1.fastq.gz	Fastqsanger.gz
	SRR31192920_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

Human cortical organoids. Chronic treatment with GSK3 inhibitor CHIR99021. Bulk RNAseq.

Project description:Here we used human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Cortical organoids were differentiated as previously described (Paşca et al., 2015, doi: 10.1038/nmeth.3415.).Chronic GSK3 inhibition was performed by adding CHIR99021 (Merck SML1046) to the medium at day 0 (1 microM) and kept throughout the differentiation process until reaching the respective collection timepoints (day 18, day 50, day 100).

2020-06-04 | E-MTAB-8325 | biostudies-arrayexpress

Single cell RNAseq of human cortical organoids investigating the effect of chronic treatment with GSK3 inhibitor CHIR99021

Project description:Here we used human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Cortical organoids were differentiated as previously described (Paşca et al., 2015, doi: 10.1038/nmeth.3415.). Chronic GSK3 inhibition was performed by adding CHIR99021 (Merck SML1046) to the medium at day 0 (1 microM) and kept throughout the differentiation process until reaching the respective collection timepoints (day 50, day 100).

2020-06-09 | E-MTAB-8337 | biostudies-arrayexpress

Lis1 in human cortical organoids

Project description:Lysate analysis of WT or Lis1-mutant human cortical organoids

2025-03-11 | PXD040813 | Pride

EVOLUTION OF THE LIPIDOME UNCOVERS EARLY CHANGES IN ADRENOLEUKODYSTROPHY HUMAN CORTICAL AND SPINAL ORGANOIDS

Project description:<p>Lipids are critical for the structure, signaling, and metabolism of the central nervous system (CNS), yet their roles during human brain development remain underexplored due to limited tissue availability. X-linked adrenoleukodystrophy (ALD), a peroxisomal disorder caused by ABCD1 mutations, disrupts very long-chain fatty acid (VLCFA) degradation, leading to axonal degeneration and demyelination. To investigate lipid dynamics in CNS development and ALD pathogenesis, we generated human induced pluripotent stem cell (hiPSC)-derived cortical and spinal cord organoids and performed lipidomics over 200 days. Lipidomic analysis revealed a dynamic lipidome, with changes in lipid abundance, saturation, and chain length reflecting neurodevelopment. ALD hiPSC-derived organoids exhibited significant lipid alterations over time, including elevated VLCFA levels and reductions in brain-relevant lipids, such as sulfatides and gangliosides, in cortical organoids. These findings provide a foundational resource for studying lipid dynamics in CNS development and emphasize the value of organoids for understanding ALD and other CNS diseases.</p>

2025-11-17 | MTBLS12973 | MetaboLights

Modeling Focal Cortical Dysplasia (FCD) using cortical organoids

Project description:We generated cortical organoids from four FCD patients. To generate cortical organoids, we used induced pluriplotent stem cells (iPSCs) obtained from skin biopsy from these FCD selected patients and healthy controls. We extrated RNA samples from the cortical organoids to do customized panel of gene expression. Gene expression using NanoString Human Neuropathology Panel from four FCD patients and four controls

2022-01-01 | GSE174605 | GEO

Modeling Focal Cortical Dysplasia (FCD) using cortical organoids

Project description:Modeling Focal Cortical Dysplasia (FCD) using cortical organoids

| PRJNA730718 | ENA

Proteomics characterisation of induced pluripotent stem cells and cortical brain organoids.

Project description:A method was developed to reproducibly produce neural retina and cortical brain regions from confluent cultures of stem cells. The spontaneously generated cortical organoids were isolated and cultured in suspension conditions for maturation. Proteomic analysis of both the original induced pluripotent stem cells and the cortical organoids demonstrated the increased presence of synaptic components, indicating maturity.

2022-05-06 | PXD025933 | Pride

LC MS/MS of kidney organoids during differentiation from day 21 to 29

Project description:Kidney organoids are a valuable and innovative model to understand genetic diseases, kidney development and transcriptomic dynamics. However, their proteome has not been analyzed so far. Here, we analyzed the organoid proteome trajectory during differentiation. Genes involved in podocytopathies and cystic kidney diseases were abundantly expressed on protein level, distinguishing organoids from almost every available cell culture model. On their pathway to terminal differentiation, organoids developed increased deposition of extracellular matrix. Single cell transcriptomic analysis suggests that most changes locate to podocytes and early podocyte progenitors. This matrix deposition is different from commonly used animal models of glomerular disease. We grew organoids from two independent batches according to the Freedman protocol, and performed proteomic profiling (Freedman, Brooks et al. 2015, Czerniecki, Cruz et al. 2018). The IPSCs were differentiated for a three-week period until first spheroids from. From day 21 of the culture they were used in our experiments up until day 29, where off-target differentiation of organoids becomes an issue.

2023-05-12 | PXD029716 | Pride

Characterising day 90 human C9ORF72-ALS/FTD cerebral organoids at single cell level

Project description:Characterising day 90 human C9ORF72-ALS/FTD cerebral organoids at single cell level

| PRJNA1100550 | ENA

Gene expression profilling of day 30 cerebral organoids at the single cell level

Project description:Gene expression profilling of day 30 cerebral organoids at the single cell level

| PRJNA1180579 | ENA