Project description:To investigate the underlying mechanism of shikonin in TNBC, we performed high-throughput sequencing to screen differentially expressed genes 24 hours after shikonin treatment
Project description:Targeted interference of sin3a-tgif1 function by SID decoy treatment inhibits WNT signaling and invasion in triple negative breast cancer cells. MDA-MB-231 cells were treated with scrambled SID control, 2.5µM SID peptide or untreated for 24h. Sub-confluent cultures of MDA-MB-231 cells were treated with scrambled SID control, 2.5µM SID peptide or untreated for 24h. Experiments were performed as three independent replicates.
Project description:Targeted interference of sin3a-tgif1 function by SID decoy treatment inhibits WNT signaling and invasion in triple negative breast cancer cells. MDA-MB-231 cells were treated with scrambled SID control, 2.5µM SID peptide or untreated for 24h.
Project description:After over 25cycle and 18month's induction of shikonin, K562 cell show maginal resistance to shikonin but great change in gene expression We use microarray to detect the global gene expression change of shikonin resistant cell
Project description:To investigate the role of shikonin in the development of colon cancer, we treated HCT116 cells with shikonin for 24 hours We then used RNA-seq data from HCT116 cells for gene expression profiling
Project description:To identify target genes of microRNA-449a in triple negative breast cancer (TNBC) we transfected the TNBC cell line HCC38 with miRNA449a mimics.