Project description:JIB-04 is an inhibitor of Jumonji histone demethylases identified through a cell based screen that measures the reactivation of an epigenetically silenced transgene. The active JIB-04 E-isomer shows selectivity for cancer vs. normal cells affecting both transcriptional patterns and cell viability in a cancer specific manner. H358 lung cancer cells or the patient matched HCC4017 (cancer) vs. HBEC30KT (immortalized normal) lung cell pair were treated with DMSO vehicle or 500nM E-isomer or 500 nM Z-isomer of JIB-04 for 4 h or 24 h and RNA extracted.
Project description:We report mapping of the PAX8 cistrome in three high grade serous ovarian cancer cell lines (KURAMOCHI, OVSAHO, and JHOS4) compared to three benign immortalized fallopian tube cell lines (FT33, FT194, and FT246). We identified a highly conserved PAX8 binding pattern common across benign fallopian tube cell lines that was distinct from the unique PAX8 binding patterns seen in each cancer cell line. Comparison of benign and malignant Mullerian cell lines with and without PAX8 knockdown. For each cell line, three distinct siRNAs targeting PAX8 plus a pool of all three siRNAs were examined and compared to both a non-transfected control as well as a control transfected with a non-targeting siRNA.
Project description:OCI_Ly1 cells treated with either DMSO or two different concentrations (150nM and 500nM) of the KDM4 inhibitor, JIB-04 were used for RNA-seq
Project description:OCI_Ly1 cells treated with either DMSO or two different concentrations (150nM and 500nM) of the KDM4 inhibitor, JIB-04 were used for chip-seq
Project description:JIB-04 is an inhibitor of Jumonji histone demethylases identified through a cell based screen that measures the reactivation of an epigenetically silenced transgene. The active JIB-04 E-isomer shows selectivity for cancer vs. normal cells affecting both transcriptional patterns and cell viability in a cancer specific manner.
Project description:Global transcriptomic profiling of JIB-04 treated and untreated control early gametocytes to determine the downstream effects of Jumonji histone demethylase inhibition on development
Project description:Single end 3' RNA sequencing of embryonal carcinoma cell line 2102EP treated with epigenetic agents Quisinostat, JIB-04, Chaetocin, Mz-1, LP99, PRT4165 and GSK343. DMSO was used as solvent control. Cells were treated with the epi-drugs for 16 h.
Project description:Our RNA sequencing analysis revealed that the JIB-04 treatment altered the expression of genes that are involved in the cell cycle, p53 signaling pathway, and apoptosis, and are also related to several cancers including hepatocellular carcinoma. JIB-04 also altered the expression of genes involved in various signaling pathways such as the FoxO signaling pathway, the PI3K-Akt signaling pathway, which is crucial for the proliferation and maintenance of hepatocellular carcinoma cells.
Project description:To examine the role of CPNE8 and BHLHE41 in ovarian cancer cells, RMG1 cells were treated with siRNA targeting CPNE8 (siCPNE8 #A and #B) or control siRNA (siControl), and OVCAR3 cells were treated with siRNA targeting BHLHE41 (siBHLHE41 #A and #B) or control siRNA (siControl). Microarray analysis showed that CPNE8 and BHLHE41 are involved in cellular signaling pathways in ovarian cancer.
