Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1184791 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR31326900_1.fastq.gz | Fastqsanger.gz | |||
| SRR31326900_2.fastq.gz | Fastqsanger.gz | |||
| SRR31326901_1.fastq.gz | Fastqsanger.gz | |||
| SRR31326901_2.fastq.gz | Fastqsanger.gz | |||
| SRR31326902_1.fastq.gz | Fastqsanger.gz |
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Project description:Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics and proteomics approach. Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrient availability, leading to protein degradation through the proteasome (PSMD2), with resulting amino acids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulation (iodine and bromine) contributed to increased oxidative stress, evident from elevated levels of antioxidant proteins like catalase, as a response to maintain tissue homeostasis. The upregulation of the gene AQP7, associated with the osmotic stress response, further supports these findings. Consequently, an increase in chaperone activity, including HSP90, was required to repair damaged proteins. Our results suggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactuca alone, potentially due to side effects arising from cell wall degradation during digestion.
2024-02-27 | PXD043394 | Pride
Project description:Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics (RNA-seq) and proteomics (LC-MS) approach.
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Project description:Weaning is a very critical period for piglets, typically accompanied by lower feed intake, weight loss after weaning and increased mortality. At weaning, piglets are exposed to many stressors, such as loss of mothering, mixing with other litters, end of lactational immunity, and a change in their environment and gut microbiota. After weaning, morphological and histological changes occur in the small intestine of piglets producing a rapid change of feeding regime which is critical for the immature digestive system. Sixteen female piglets were weaned to assess the effect of sorbic acid supplementation on the small intestine tissue transcriptome. At weaning day (T0), 4 piglets were sacrified and tissue samples collected. The remaining 12 piglets were weighted and randomly assigned to different post weaning (T5) diets. Diet A (n=6) contained 5 g/kg of sorbic acid. Diet B (n=6) is the same as Standard diet. Total RNA was isolated from ileum samples to be analyzed using the a CombiMatrix CustomArrayTM 90K platform . Even though diet had no detectable effect during the first 5 days after weaning, outcomes from this study highlighted some of the response mechanisms to the stress of weaning occurring in the piglet gut. A total of 205 differentially expressed genes were used for functional analysis using bioinformatics through BLAST2GO, Ingenuity Pathway Analysis 8.0, and the Dynamic Impact Aproach (DIA). Bioinformatics analysis revealed that Apoptosis, RIG-I-like and NOD-like receptor signaling were altered as a result of weaning. Results suggest that immune and inflammatory responses were activated and likely are a cause of small intestine atrophy as revealed by a decrease in villus height and villus/crypt ratio. Keywords: weaning, gut, gene expression, sorbic acid, microarray analysis
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Project description:Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed,food, and fuel. They can also contribute to the improved development of weaned piglets. However,their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed throughcarbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study wasto assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyasesupplementation, using an integrated transcriptomics (RNA-seq) and proteomics (LC–MS) approach.Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrientavailability, leading to protein degradation through the proteasome (PSMD2), with resulting aminoacids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulationmay have contributed to increased oxidative stress, evident from elevated levels of antioxidantproteins like catalase, as a response to maintaining tissue homeostasis. The upregulation of the geneAQP7, associated with the osmotic stress response, further supports these findings. Consequently, anincrease in chaperone activity, including HSP90, was required to repair damaged proteins. Our resultssuggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactucaalone, potentially due to side effects of cell wall degradation during digestion.
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