Project description:This study provides a comprehensive genomic characterization of Streptococcus oralis CRC211, a novel bacterial strain isolated from colorectal tumor tissue, through whole-genome sequencing and comparative analysis. The high-quality assembled genome (15.03 Mb, 40.94% GC content) contains 2 prophage regions spanning 160.5 kb, which may facilitate horizontal transfer of virulence genes. Functional annotation identified 3,674 genes, with significant enrichment in metabolic pathways (amino acid and carbohydrate metabolism) and virulence factors (116 genes in VFDB), including adhesins and biofilm-associated proteins that likely promote tumor colonization. Comparative genomic analysis revealed CRC211 shares 92.29% average nucleotide identity with reference S. oralis strains, while pan-genome analysis demonstrated an open genome structure with 1,222 conserved core genes. The strain also carries 75 antimicrobial resistance genes, suggesting potential clinical relevance. Notably, the genomic profile indicates adaptations for nutrient acquisition and immune evasion in the tumor microenvironment. These findings establish CRC211 as a CRC-associated strain with distinct genomic features that may contribute to tumor progression, providing crucial insights for future investigations into its oncogenic mechanisms and potential applications in microbiota-based diagnostics or therapeutics for colorectal cancer.
Project description:We compared the gene expression patterns of macrophages infected with S. oralis wild type and SpxB KO, a strain that does not produce H2O2.
Project description:The zur regulon in Neisseria meningitidis was elucidated in the strain MC58 using a zur knockout strain and conditions which activate Zur ( zinc supplementation in the medium)
Project description:Investigation of whole genome gene expression level changes in S. pneumoniae KCTC 5080T, S. mitis KCTC 3556T, S. oralis KCTC 13048T, and S. pseudopneumoniae CCUG 49455T. This proves that transcriptional profiling can facilitate in elucidating the genetic distance between closely related strains. A one chip study using total RNA recovered from S. pseudopneumoniae CCUG 49455T with three strain. For the the transcriptome of S. pseudopneumoniae CCUG 49455T was analyzed using the S. pneumoniae R6 microarray platform and compared with those of S. pneumoniae KCTC 5080T, S. mitis KCTC 3556T, and S. oralis KCTC 13048T strains.