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ABSTRACT:
PROVIDER: PRJNA1193706 | ENA |
REPOSITORIES: ENA
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Project description:Polygonatum odoratum (MILL.) DRUCE is rich in bioactive components with high medicinal value. To maximize the clinical benefits, it is of great significance to efficiently extract key bioactive components from appropriate growth stages in which they are most abundant. In this study, we analyzed the changes of metabolite accumulation and protein expression in P. odoratum rhizomes at different growth stages using targeted metabolomics combined with proteomics, and identified a total of 1,237 differentially abundant metabolites (DAMs). Flavonoids accumulated most in winter, and the biosynthesis pathways associated with flavonoids, isoflavonoids, flavones and flavonols exhibited significant differentially expressed proteins (DEPs). Among them, PGT, FLS, CYP75B1, HIDH, IF7MAT, and UFT73C6 were positively correlated with flavonoid accumulation. Steroid saponins accumulated most in spring, and the biosynthetic pathways of steroid and brassinosteroid biosynthesis exhibited DEPs. Among them, FDFT1, TM7SF2, DHCR7, CAS1, and 3BETAHSDD were positively correlated with steroidal saponin accumulation. In summary, these results revealed the accumulation of secondary metabolites P. odoratum in different growth stages, which can provide an effective reference for the extraction of specific bioactive components and the study of their regulatory mechanisms.
2024-12-13 | PXD058889 |
Project description:Polygonatum odoratum (MILL.) DRUCE is rich in bioactive components with high medicinal value. To maximize the clinical benefits, it is of great significance to efficiently extract key bioactive components from appropriate growth stages in which they are most abundant. In this study, we analyzed the changes of metabolite accumulation and protein expression in P. odoratum rhizomes at different growth stages using targeted metabolomics combined with proteomics, and identified a total of 1,237 differentially abundant metabolites (DAMs). Flavonoids accumulated most in winter, and the biosynthesis pathways associated with flavonoids, isoflavonoids, flavones and flavonols exhibited significant differentially expressed proteins (DEPs). Among them, PGT, FLS, CYP75B1, HIDH, IF7MAT, and UFT73C6 were positively correlated with flavonoid accumulation. Steroid saponins accumulated most in spring, and the biosynthetic pathways of steroid and brassinosteroid biosynthesis exhibited DEPs. Among them, FDFT1, TM7SF2, DHCR7, CAS1
2024-12-14 | PXD058887 |
Project description:Polygonatum odoratum Transcriptome or Gene expression
| PRJNA1281364 | ENA
Project description:Integrated transcriptomic and metabolomic analysis to reveal the mechanism of ischemic tolerance in TIA patients
| PRJNA1007636 | ENA
Project description:Root rot disease poses a devastating threat to Coptis chinensis Franch, a medicinal plant prized for its bioactive alkaloids. To dissect its defense mechanisms, we conducted integrated transcriptomic and metabolomic analyses on resistant (R), early-stage infected (S-ES), and late-stage infected (S-LS) plants. Our findings reveal a disease severity-dependent escalation in flavonoid metabolism. Key metabolites, such as kaempferol and quercetin derivatives, were significantly increased compared to R, paralleled by progressive upregulation of biosynthetic genes (PAL, CHS, CHI, FLS). Strikingly, salicylic acid (SA)-associated metabolites and pathway genes (NPR1, NPR3/NPR4) showed no differential expression across groups, contrasting with typical SA-mediated defenses in other species. This study uncovers flavonoid biosynthesis as the primary defense strategy in C. chinensis during root rot progression, while SA signaling may not be the main defense mechanism. These results provide actionable targets for enhancing disease resistance in medicinal plants through metabolic engineering
2025-03-25 | GSE290673 | GEO