Comprehensive analysis of microRNA after moderate intensity exercise in mice
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PROVIDER: PRJNA1195028 | ENA |
REPOSITORIES: ENA
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2025-01-29 | GSE283677 | GEO
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2025-02-10 | PXD053276 | Pride
Project description:Physical exercise is beneficial to keep physical and mental health. The molecular mechanisms underlying exercise are still worth exploring.The healthy adult mice after six weeks of moderate-intensity exercise (experimental group) and sedentary mice (control group) were used to perform transcriptomic, proteomic, lactylation modification, and metabolomics analysis. In addition, gene sets related to hypoxia, glycolysis, and fatty acid metabolism were used to aid in the screening of hub genes. The mMCP-counter was employed to evaluate infiltration of immune cells in murine liver tissues.
2025-01-02 | GSE280324 | GEO
Project description:While exercise is widely recognized to enhance bone mass, the underlying molecular and cellular mechanisms remain incompletely elucidated. The present study was designed to investigate the effect of moderate-intensity exercise on skeletal quality, morphological characteristics, and metabolic homeostasis in adult Sprague-Dawley (SD) rats. Herein, we aim to investigate the expression profile of mRNAs in rats with exercise and identify promising targets for osteoporosis diagnosis and treatment. RNA sequence were performed in the bones from 3 exercised rats and 3 healthy controls.
2026-01-07 | GSE310294 | GEO
Project description:To explore the effects of moderate intensity exercise on protein of lactylation in mouse muscle tissue metabolism. The healthy adult mice running for 6 weeks as exercise model and sedentary mice as control were used to perform transcriptomic, proteomic, lactylation-proteomics, and metabolomics analysis. In addition, correlation analysis between transcriptome and proteome, and proteome and metabolome was conducted as well.
2024-08-21 | GSE270280 | GEO
Project description:Exercise is recognized as a first-line lifestyle therapy for many cardiometabolic diseases, including obesity, type 2 diabetes, and hypertension. Despite the abundant health-promoting effects of exercise, in-depth characterization of circulatory factors mediating these benefits remain understudied in humans. Moreover, how different modes of exercise uniquely regulate these processes is unclear. Here, we address these questions by conducting a multi-cohort human exercise intervention, incorporating sprint-interval exercise (SIE), moderate-intensity exercise (MIE), and treadmill exercise to exhaustion to analyze intensity-dependent regulation of adipocytes and bulk adipose tissue. We find that exercise intensity uniquely influences the plasma proteome and plasma metabolome, and culturing primary human adipocytes with these plasma samples results in robust intensity-dependent transcriptome changes. We confirm many of these genes are similarly regulated by intense exercise in vivo, via RNA-sequencing of bulk adipose tissue following treadmill exercise to exhaustion. These findings underscore intensity-dependent regulation of adipose tissue following exercise.
2026-06-23 | GSE308252 | GEO
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2021-11-17 | GSE184493 | GEO
Project description:The primary aim of this study was to compare the acute effects of three exercise intensities on abdominal subcutaneous adipose tissue (aSAT) transcriptome in regular exercisers. A total of 45 adults who exercise regularly were assigned to perform a single session of either low-intensity continuous (LOW; 60min at 30% VO2max; n=15), moderate-intensity continuous (MOD; 45min at 65% VO2max; n=15), or high-intensity interval exercise (HIGH; 10x1min at 90% VO2max interspersed with 1min active recovery; n=15). aSAT biopsy samples were collected before and 1.5hours after the exercise session for bulk RNA sequencing and targeted protein immunoassays. HIGH upregulated genes involved in cytokine secretion, insulin signaling, and proteolysis while MOD and LOW upregulated genes regulating ECM remodeling, ribosome biogenesis, and oxidative phosphorylation pathways. Exercise-induced changes in aSAT angiogenic, MAPK cascade, and clock genes, ERK protein phosphorylation, and circulating cytokines were similar after all three exercise treatments. Network analysis identified exercise-responsive gene clusters linked to cardiometabolic health traits. Cell-type analysis highlighted a heterogeneous response of aSAT cell types to exercise, with distinct patterns observed across exercise intensities. Collectively, our data characterizes early responses in aSAT after a single session of exercise. Because adaptations to exercise training stem from an accrual of responses after each session of exercise, these early responses to exercise are likely important contributors to the long-term structural and functional changes that occur in adipose tissue in response to exercise training.
2025-10-21 | GSE303983 | GEO
Project description:Moderate-intensity exercise affects gut microbiome
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Project description:Sprint interval exercise (SIE) induces mitochondrial adaptations in skeletal muscle, comparable to or greater than moderate-intensity continuous exercise (MICE), despite lower training volume. These effects are partly due to enhanced mitochondrial bioenergetics, including increased Ca²⁺ uptake and pyruvate dehydrogenase (PDH) activation. Oleuropein (OLE), a compound from olive leaf extract, also promotes mitochondrial Ca²⁺ uptake and PDH activation in mice. The current study tested whether OLE could enhance these adaptations in humans during MICE or SIE. In a double-blind crossover design, healthy males performed either MICE or SIE with OLE supplementation. To elucidate the acute molecular response of skeletal muscle following exercise, we performed mass spectrometry-based proteomic analysis.
2026-05-13 | PXD072766 | Pride