Project description:Transcriptional profiling of Caco-2 cells comparing Caco-2 monolayers cultured in a custom built co-culture chamber, either inside a 5% CO₂ incubator (conventional cell culture environment) or an anaerobic workstation (apical anaerobic environment) for 12 hours.
Project description:Polyphosphate accumulating organisms are responsible for enhanced biological phosphate removal from wastewater, where they grow embedded in a matrix of extracellular polymeric substances. Little is known about the composition and dynamics of those proteins and their production by the different microorganisms. Tomás-Martínez et al., (2022) studied the turnover of proteins and polysaccharides in extracellular polymeric fractions of an enrichment culture of polyphosphate accumulating organisms using an anaerobic-aerobic sequencing batch reactor simulating EBPR conditions. Finally, the carbon source was switched to 13C-labelled acetate to study the protein turnover. Samples were collected at the end of each aerobic phase.
Project description:Interventions: Analysis of bacteremia after ESD of the colon.
Primary outcome(s): Identification of bacteremia after ESD testing blood culture and 16SrRNA gene sequencing.
Study Design: Single arm Non-randomized
Project description:Transcriptional profiling of Paracoccus denitrificans PD1222 wild type incubated in continuous culture (continuous culture (CSTR)) in minimal media with aerobic or anaerobic conditions. The goal was to define the core respiratory genes.
Project description:The anaerobic gut microbe Akkermansia muciniphila ATCC BAA-835 lives in the mucus layer where it is exposed to oxygen. To investigate how it survives the changing oxygen concentrations, we exposed a exponentially growing culture to oxygen. The experiment was performed in parallel fermentor systems. To one system we added 0.2l/h oxygen after the culture reached an OD of 0.1, while the other remained anaerobic. Samples were taken just before addition of oxygen, about 1h after and when the cell reached stationary phase.
Project description:The differential RNA-seq data contained within this entry is complemented by global RNA-seq and microarray data, which is also deposited in GEO. Duplicate cultures of Propionibacterium acnes strain KPA171202 were grown exponentially in batch culture under anaerobic growth. Samples were taken following subculture with and without potassium downshift (i.e. removal from medium). This produced 4 samples; 2 replicates x 2 conditions. Aliquots of each of the 4 samples were then incubated with or without incubation TAP (tobacco acid pyrophosphatase) before library construction. Thus, 8 libraries were analysed. TAP treatment allows the cloning and sequencing of 5' ends that were originally triphosphorylated.
Project description:In this study, we used an original system to produce C. difficile anaerobic biofilms consisting in continuous-flow microfermentors, that is compared to planktonic growth culture.
Project description:This study characterizes the development of an anaerobic intestinal cell culture model based on a dual flow chamber system using Caco-2 cells. One goal of the study was to characterize the transcriptomic profile of Caco-2 cells grown in the anaerobic dual flow chamber system and characterizing the effect of oxygen- and flow conditions on the maturation of the cells.
2025-08-04 | GSE284670 | GEO
Project description:Sequencing of anaerobic environmental samples
