Project description:Mesenchymal stromal cells belong to a diverse collection of cells in different states that are poorly characterized in soft-tissue sarcomas. We used microarrays to explore differentially expressed genes in pediatric rhabdomyosarcoma cells (RD) after exposure to non-malignant mesenchymal stromal cells (4 replicates/condition).
Project description:RD induced DNA damage and suppressed DNA repair in PC-3 cells. Expression profiling of PC-3 cells treated with 10 umol/L RD for 24 hours.
Project description:To analyze gene expression of RD cells after treatment with an anti-picornavirus compound MDL-860, we have employed whole genome microarray expression profiling as a discovery platform. Human RD cells were treated with mock or MDL-860 (final concentration of 20 uM) for 24 hours. A cluster of genes regulated Nrf2-Keap1 antioxidant pathway were identified by gene ontology analysis.
Project description:To investigate the effect of a particular gene on CVA6 replication, we established a CVA6-infected RD cell line. We then performed gene expression analysis using RNA-seq data of cells from the infected and uninfected groups.
Project description:RD induced DNA damage and suppressed DNA repair in PC-3 cells. Expression profiling of PC-3 cells treated with 10 umol/L RD for 24 hours. Total RNA was extracted from PC-3 cells exposed to RD for 24 h using an RNAiso plus kit (TaKaRa). Microarray analysis was performed on separate samples on the Affymetrix HG-U133 plus 2 chip.
Project description:Comparing the gene expression profiling of HDGF-silenced RD-ES cells and control RD-ES cells to identify genes regulated by HDGF in RD-ES cells. Keywords: expression analysis
Project description:Transcriptome analysis in patients with IgG4-RD and healthy controls IgG4-related disease (IgG4-RD) is a new emerging disease entity characterized by elevated serum IgG4 concentrations and tissue tumefaction or infiltration by IgG4-positive plasma cells. At present, however, it is not clear whether IgG4-RD is caused by abnormalities in acquired immunity, like autoimmune diseases, or whether the excess production of IgG4 is a true cause of IgG4-RD or an epiphenomenon associated with inflammatory and/or allergic reactions. We therefore attempted to identify genes related to the pathogenesis or clinicopathology of IgG4-RD. Peripheral blood mononuclear cells (PBMCs) were obtained from patients with IgG4-RD before and after steroid therapy and from healthy controls. Total RNA was extracted and DNA microarray analysis was performed to screen for genes showing changes in expression.
