Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1196009 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Aestivation is a dormant state that allows animals to withstand hot and dry summer conditions and requires complex gene regulation. Nevertheless, the mechanisms involved in the regulation of genes necessary for aestivation remain unclear. MicroRNA (miRNA) are known to fine-tune gene expression at the post-transcriptional level and are important for various biological processes. In this study, we investigated the role of the miRNA pathway in the regulation of the obligatory aestivation stage in the cabbage stem flea beetle, a major pest of oilseed rape. Small RNA sequencing showed that ∼25% of miRNAs were differentially abundant during aestivation. The inhibition of the miRNA pathway deregulated 116 proteins in aestivation, which were mainly associated with metabolism and catabolism, including peroxisome activity. Most proteins regulated by miRNA exhibited lower transcript levels during aestivation. RNA degradome sequencing confirmed the miRNA-mediated exonucleolytic decay of several transcripts. Furthermore, inhibiting the miRNA pathway resulted in altered body composition, compromised metabolic suppression, and lower resilience to high temperature during aestivation. Also, beetles could not suppress their feeding activity during the transition into aestivation. Our findings highlight the critical role of miRNA in regulating aestivation in the cabbage stem flea beetle, with important implications for climate change.
2025-07-26 | PXD061878 | Pride
Project description:Populus deltoides ‘Shalinyang’ (PdS), a vital species for artificial shelterbelts in the arid regions of northwest China, has shown resistance to the destructive stem-boring beetle Anoplophora glabripennis Motschulsky (Asian longhorned beetle, ALB). However, comprehensive research in this area is still lacking. This study compared PDS leaves and phloem tissues before and after ALB adult feeding, utilizing untargeted metabolomics (LC-MS/MS) and transcriptomics (RNA-Seq) methods. The investigation identified a total of 3,552 metabolites across the 12 samples. Transcriptomic sequencing generated 82.52 Gb of clean data, with an average of 6.88 Gb per sample, and annotated 33,429 expressed genes. These data provide fundamental and valuable information for understanding how the resistant species PdS responds to ALB adult infestation. Furthermore, they can be utilized in future studies to explore the interaction mechanisms between poplar species and ALB and investigate the insect resistance mechanisms of PdS.
2026-03-25 | MTBLS12296 | MetaboLights
Project description:The striped flea beetle (SFB), Phyllotreta striolata, is a globally devastating cruciferous pest. A key challenge to its control lies in its complex life cycle—with egg, larval, and pupal stages confined to soil, while adults infest aerial plant parts—coupled with distinct antennal differences between males and females that underpin sex-specific ecological traits. Transcriptomic profiling across developmental stages offers a powerful approach to decode the genetic basis of these stage- and sex-related traits and inform targeted pest management. Here, we present the first comprehensive transcriptomic atlas of P. striolata, encompassing four key life stages (egg, larva, pupa, adult) with separate male and female adult. RNA sequencing of 15 samples (biological triplicates per group) generated 334.5 million raw reads. Dataset reliability was robust, as validated by correlation analysis, principal component analysis, and qRT-PCR. Differential expression analysis and functional enrichment further confirmed the dataset’s utility in dissecting stage-specific developmental programs and sex-biased molecular signatures. These data provide valuable insights into the biology and developmental dynamics of P. striolata and lay a solid foundation for functional genetic studies, thereby facilitating the identification of target genes and the development of effective, sustainable control strategies against this pest.
2026-07-22 | GSE338987 | GEO
Project description:Plants possess various defense strategies to counter attacks from microorganisms or herbivores. For example, plants reduce the cell-wall-macerating activity of pathogen- or insect-derived polygalacturonases (PGs) by expressing PG-inhibiting proteins (PGIPs). PGs and PGIPs belong to multi-gene families believed to have been shaped by an evolutionary arms race. The mustard leaf beetle Phaedon cochleariae expresses both active PGs and catalytically inactive PG pseudoenzymes. Previous studies demonstrated that (i) PGIPs target beetle PGs and (ii) the role of PG pseudoenzymes remains elusive, despite having been linked to the pectin degradation pathway. For further insight into the interaction between plant PGIPs and beetle PG family members, we combined affinity purification with proteomics and gene expression analyses, and identified novel inhibitors of beetle PGs from Chinese cabbage (Brassica rapa ssp. pekinensis). A beetle PG pseudoenzyme was not targeted by PGIPs, but instead interacted with PGIP-like proteins. Phylogenetic analysis revealed that PGIP-like proteins clustered apart from classical PGIPs but together with proteins, which have been involved in developmental processes. Our results indicate that PGIP-like proteins represent not only interesting novel PG inhibitor candidates in addition to classical PGIPs, but also fascinating new players in the arms race between herbivorous beetles and plant defenses.
2021-06-07 | PXD022562 | Pride
Project description:Purpose: we want to see gene expression changes during in vitro expansion of VM-derived NSCs (VM-NSCs) with cell passges in the absence or presence of Lin28a overexpression. changes upon Lin28 overexpression in P1 and P3 stages of Neural stem cells. RNA-seq, sRNA-seq, and Polysome-seq with/without Lin28 overexpression in P1 and P3 stages of Neural stem cells.
2016-08-11 | E-GEOD-77851 | biostudies-arrayexpress