Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes) and developing seeds. This study compare the gene expression profile in wheat spikelets (spk 2) inoculated with either water (mock treatment) or a pathogenic strain of Fusarium graminearum (WT); spikelets 2 were inoculated 24 hrs after a neighbour spikelet (spk 0) was treated with either water or F. graminerum mutant strain Tri6Δ or NoxABΔ. Spikelets 2 were sampled 8 and 24 hrs after the second treatment.
Project description:Purpose: To assess global changes of fusarium graminearum gene expressional responses to different nitrogen source and gene expression changes after removal different histone methylation hallmarks. Methods: For RNA seq of fusarium, three biological replicates of hyphae after 48/72 hours cultured were sequenced. Results: For each sample, 5.83-8.31G RNA-seq clean reads were obtained mapped to the Fusarium graminearum genome sequence.
