Project description:Pokkah boeng disease, caused by the fungal pathogen Fusarium sacchari, is a significant threat to sugarcane production worldwide. To elucidate the molecular defense mechanisms of sugarcane against this pathogen, we performed a comprehensive transcriptomic analysis using the hybrid cultivar ZZ1 (Saccharum officinarum x Saccharum spontaneum). Leaf tissues were collected at multiple time points (0, 12, 24, 48, 72, 120, and 144 hours) following inoculation with F. sacchari. Total RNA was extracted and sequenced using the Illumina Novaseq platform. This study aims to identify key differentially expressed genes and regulatory pathways involved in the sugarcane-pathogen interaction, providing valuable genomic resources for breeding disease-resistant sugarcane varieties.
Project description:Few aerobic hyperthermophiles degrade polysaccharides. We describe the genome-enabled enrichment and isolation of an aerobic hyperthermophile, Fervidibacter sacchari, which was originally ascribed to candidate phylum Fervidibacteria. F. sacchari uses polysaccharides and monosaccharides as sole carbon sources from 65-87.5 °C, and its genome encodes 117 glycoside hydrolases (GHs) spanning 49 GH families, including 31 homologs of understudied GH109, GH177, and GH179 domains. Here, we analyzed the transcriptomes of F. sacchari cells grown on eight different sole carbon and energy sources (beta-glucan, chondroitin sulfate, corn stover, gellan gum, locust bean gum, starch, xanthan gum, and xyloglucan) to link glycoside hydrolase substrate to function, as well as identify potential regulatory mechanisms. These data will provide preliminary characterization of novel carbohydrate-active enzymes at high temperatures.