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Gene expression profile of embryonic stem cell-derived TS-like cells by single cell RNA-sequencing

ABSTRACT: Gene expression profile of embryonic stem cell-derived TS-like cells by single cell RNA-sequencing

PROVIDER: PRJNA1198541 | ENA |

REPOSITORIES: ENA

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Gene expression profile of embryonic stem cell-derived TS-like cells by RNA-sequencing

Project description:Gene expression profile of embryonic stem cell-derived TS-like cells by RNA-sequencing

| PRJNA1198534 | ENA

DNA methylation comparison of ntTS, TS and ES cells

Project description:Placental abnormalities occur frequently in cloned animals. To investigate DNA methylation profile of trophoblast cell lineage of somatic cell nuclear transferred (NT) embryos, we established TS cells from blastocysts produced by NT at the blastocyst stage. Three independent TS cell lines derived from NT embryos rom BDF1 background were used in this study. Two control lines, derived from native blastocysts of BDF1 background, were also used.

2010-05-16 | E-GEOD-17104 | biostudies-arrayexpress

Label-free quantitative analysis proteomic profiles of human embryonic stem cell derived exosomes

Project description:In order to investigate the characteristics and mechanisms of embryonic stem cell derived exosomes attenuates transverse aortic constriction induced ventricular remodeling, the proteomic profiles of human embryonic stem cell derived exosomes were analysed by label-free quantification.

2020-12-10 | PXD023105 |

Gene expression profile of embryonic stem cell-derived TS-like cells by RNA-sequencing

Project description:Human embryonic stem cells (ESCs) were differentiated into GATA3-expressing extraembryonic cells (GATA3+ ExE cells) by exposure to chemical inhibitors targeting ACTIVIN/NODAL (A83-01, 1uM) and FGF signaling (PD03714, 100nM). Using these GATA3-expressing cells as a starting material, we established trophoblast stem-like cells (TSLCs) following the established protocol for deriving trophoblast stem cells (TSCs) from the villous trophoblast of human first-trimester placenta. To characterize the resulting ESC-derived TSLCs, RNA sequencing was conducted, allowing comparisons with the parental ESCs and previously published TSCs.

2025-05-31 | GSE284316 | GEO

Gene expression profile of embryonic stem cell-derived TS-like cells by single cell RNA-sequencing

Project description:Human embryonic stem cells (ESCs) were differentiated into GATA3-expressing extraembryonic cells (GATA3+ ExE cells) by exposure to chemical inhibitors targeting ACTIVIN/NODAL (A83-01, 1uM) and FGF signaling (PD03714, 100nM). Using these GATA3-expressing cells as a starting material, we established trophoblast stem-like cells (TSLCs) following the established protocol for deriving trophoblast stem cells (TSCs) from the villous trophoblast of human first-trimester placenta. To understand the transcriptional properties of individual TSLCs, we obtained the gene expression profile from single cells.

2025-05-31 | GSE284317 | GEO

Metabolic enhancement of developmental pausing (TS cells)

Project description:Embryonic stem (ES) cells and embryos reversibly pause via chemical mTOR inhibition. In this study, we investigate the tissue-specific response to mTORi-induced pausing in ES and trophoblast stem (TS) cells. To resolve the sequential rewiring of the proteome, we conducted a time-series proteomics experiment at 1, 3, 6, 12, 24, and 48 hours upon induction of pausing, and at 1, 3, 6, 12, 24, and 48 hours upon release of pausing in ES and TS cells. We find that ES, but not TS cells pause reversibly. To optimise developmental pausing conditions, we reasoned that by understanding the difference in pausing response of ES and TS cells, we could identify which pathways are essential for pausing. We found that KEGG pathways related to amino acid degradation, fatty acid degradation, and DNA repair are upregulated in ES cells, but downregulated in TS cells during entry into pausing. Moreover, by targeted metabolomics, we found a depletion of short chain carnitines in the paused ES cells. To extend the length of developmental pausing, we supplemented paused embryos with L-carnitine. The L-carnitine supplementation facilitates lipid usage and prolongs the pausing length by 19 days through the establishment of a more dormant state.

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TS-seq

Project description:TS-seq

| PRJNA847608 | ENA

Expression profiling of ntTS and TS cells

Project description:Placental abnormalities occur frequently in cloned animals. To investigate gene expression profile of trophoblast cell lineage of somatic cell nuclear transferred (NT) embryos, we established TS cells from blastocysts produced by NT at the blastocyst stage.

2009-07-17 | GSE17102 | GEO

DNA methylation comparison of ntTS, TS and ES cells

2009-07-17 | GSE17104 | GEO

Gene expression profile of human embryonic stem cell-derived hepatocyte-like cells

Project description:The differentiation of pluripotent stem cells to hepatocyte-like cells offers the perspective of unlimited supply of human hepatocytes. However, the degree of differentiation of hepatic-like cells (HLC) remains controversial. To obtain an unbiased characterization, we performed a transcriptomics study using the Affymetrix Gene ChipÂ® HG-U133 plus 2.0, with HLC derived from embryonic and induced stem cells (ESC, hiPSC) from two different laboratories. Genome-wide gene expression profiles of ESC and HLC were compared to freshly isolated and up to 14 days cultivated primary human hepatocytes (see submission E-MTAB-3994) obtained under patient informed consent from three male donors. Three biological replicas were used for each ESC and HLC models. (associated data set: E-MTAB-3994 - Gene expression profile of human embryonic stem cell-derived hepatocyte-like cells in matrigel and recombinant laminins)

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