Project description:RNA-sequencing was preformed from RNA isolated from bacteria infected with the bacteriophage. In order to reveal the phage-host interactions between φR1-37 and Yersinia enterocolitica throughout the phage infection cycle, both the transcriptomes were scrutinized during all the stages of infection.
Project description:The enteric pathogen Yersinia enterocolitica is a the most common gram-negative zoonotic pathogen that causes a variety of gut-associated diseases named yersiniosis ranging from enteritis, watery diarrhea, mesenteric lymphadenitis to post infectious extraintestinal sequelae such as reactive arthritis human yersiniosis. The species Y. enterocolitica comprises a diverse group of about 70 serotypes of which only 11 are harmful to humans. Among the pathogenic strains are the highly mouse-virulent 1B/O:8 strains (YeO:8). This bioserotype, in particular YeO:8 strain 8081v has been used to study the pathogenesis of Y. enterocolitica using mouse infection models. However, by far the most frequent cause of human yersiniosis in Europe and Japan (>90%) is Y. enterocolitica bioserotype O:3 (YeO:3), which is also frequently found in pigs and pork products. In this study a comparative RNA-seq-based transcriptomic approach was used to identify serotype/isolate-specific differences in the transcriptome of the isolates YeO:8 8081v and YeO:3 Y1 under infection-relevant conditions. This strategy allowed the generation of the first in-depth single-nucleotide resolution transcriptome of Y. enterocolitica, revealed major differences in the temperature- and growth phase-dependent expression profiles, and led to the discovery of changes that modulate transcripts levels of important virulence-relevant traits. Additionally a comprehensive map of transcriptional start sites for Y. enterocolitica was generated using cDNA libraries based on samples enriched for primary transcripts.
Project description:We work with the bacterium Yersinia enterocolitica, a gastrointestinal pathogen. In this study we characterized a bacterial mutant strain called SOR17. We compared the protein extracts of SOR17 with that of the parental strain JB580v. Bacteria were grown in triplicate for 5 h at 37°C, and following bacterial disruption by French press, proteins were fractionated into soluble or membrane proteins. We also compared total protein extracts of bacteria grown for 16 h at 27°C. Two-dimensional electrophoresis were performed and proteins were stained with Coomassie brilliant blue. Spots whose abundance changed more than 2 fold were excised and sent to mass spectrometry for identification by either MALDI-TOF PMF or LC-MS/MS.