Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Decoding Gene Networks Governing Hypothalamic and Prethalamic Neuron Development

ABSTRACT: Decoding Gene Networks Governing Hypothalamic and Prethalamic Neuron Development

PROVIDER: PRJNA1199406 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR31751000_1.fastq.gz	Fastqsanger.gz
	SRR31751000_2.fastq.gz	Fastqsanger.gz
	SRR31751001_1.fastq.gz	Fastqsanger.gz
	SRR31751001_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 161

Similar Datasets

Decoding Gene Networks Governing Hypothalamic and Prethalamic Neuron Development

Project description:Purpose: sc/snRNA-Seq and scATAC-Seq were performed to characterize the developing hypothalamus

2025-05-26 | GSE284492 | GEO

ATAC-seq of iPSC-derived hypothalamic arcuate-like neuron cells

Project description:We performed ATAC-seq on iPSC-derived hypothalamic arcuate-like neuron cells to identify putative regulatory elements. All samples were derived from the same individual and from the same differentiation/cell line but ATAC-seq was performed in 3 separate experiments (3 technical replicates).

2021-04-01 | E-MTAB-10257 | biostudies-arrayexpress

Decoding the molecular complexity governing corneal wound closure in vivo

Project description:Decoding the molecular complexity governing corneal wound closure in vivo

| PRJNA1238787 | ENA

Type 2 diabetes risk gene Dusp8 is a gatekeeper for hypothalamic Jnk signaling and insulin sensitivity

Project description:Recent genome-wide association studies (GWAS) identified Dusp8, a dual-specificity phosphatase targeting MAP kinases, as type 2 diabetes risk gene. Here, we unravel Dusp8 as gatekeeper in the hypothalamic control of glucose homeostasis in mice and humans. Male but not female Dusp8 loss-of-function mice, either with global or CRH neuron-specific deletion, had impaired systemic glucose tolerance and insulin sensitivity when exposed to high-fat diet (HFD). Mechanistically, we found impaired hypothalamic–pituitary–adrenal (HPA) axis feedback, blunted sympathetic responsiveness, and chronically elevated corticosterone levels driven by hypothalamic hyperactivation of Jnk signaling. Accordingly, global Jnk1 ablation, AAV-mediated Dusp8 overexpression in the mediobasal hypothalamus, or metyrapone-induced chemical adrenalectomy rescued the impaired glucose homeostasis of male Dusp8 KO mice, respectively. This sex-specific and rheostatic role of murine Dusp8 in governing hypothalamic Jnk signaling, insulin sensitivity and systemic glucose tolerance was consistent with fMRI data in human volunteers that revealed an association of the DUSP8 rs2334499 risk variant with hypothalamic insulin resistance in men. In summary, our findings suggest GWAS-identified gene Dusp8 as novel hypothalamic factor that plays a functional role in the etiology of type 2 diabetes.

2020-08-17 | PXD019451 | Pride

Functional Dissection of Transcription Factor Networks Governing Haematopoietic Progenitor States

Project description:Functional Dissection of Transcription Factor Networks Governing Haematopoietic Progenitor States

| PRJNA609352 | ENA

Dissecting intracellular receptors regulated transcription networks governing human osteoblast identities

Project description:Dissecting intracellular receptors regulated transcription networks governing human osteoblast identities

| PRJNA834792 | ENA

ATAC-seq of iPSC-derived hypothalamic arcuate-like neuron cells

Project description:ATAC-seq of iPSC-derived hypothalamic arcuate-like neuron cells

| PRJEB43848 | ENA

Type 2 diabetes risk gene Dusp8 regulates hypothalamic Jnk signaling and insulin sensitivity

Project description:Recent genome-wide association studies (GWAS) identified DUSP8, a dual-specificity phosphatase targeting MAP kinases, as type 2 diabetes (T2D) risk gene. Here, we unravel Dusp8 as gatekeeper in the hypothalamic control of glucose homeostasis in mice and humans. Male but not female Dusp8 loss-of-function mice, either with global or CRH neuron-specific deletion, had impaired systemic glucose tolerance and insulin sensitivity when exposed to high-fat diet (HFD). Mechanistically, we found impaired hypothalamic-pituitary-adrenal (HPA) axis feedback, blunted sympathetic responsiveness, and chronically elevated corticosterone levels driven by hypothalamic hyperactivation of Jnk signaling. Accordingly, global Jnk1 ablation, AAV-mediated Dusp8 overexpression in the mediobasal hypothalamus, or metyrapone-induced chemical adrenalectomy rescued the impaired glucose homeostasis of obese male Dusp8 KO mice, respectively. The sex-specific role of murine Dusp8 in governing hypothalamic Jnk signaling, insulin sensitivity and systemic glucose tolerance was consistent with fMRI data in human volunteers that revealed an association of the DUSP8 rs2334499 risk variant with hypothalamic insulin resistance in men. Further, expression of DUSP8 was increased in the infundibular nucleus of T2D humans. In summary, our findings suggest the GWAS-identified gene Dusp8 as novel hypothalamic factor that plays a functional role in the etiology of T2D.

2020-08-26 | GSE112688 | GEO

Lineage-Specific Differences and Inference of Regulatory Networks Governing Human Chondrocyte Development

Project description:Lineage-Specific Differences and Inference of Regulatory Networks Governing Human Chondrocyte Development

| PRJNA801843 | ENA

Cis-regulatory architecture of human ESC-derived hypothalamic neuron differentiation aids in variant-to-gene mapping of relevant common complex traits (Capture C)

Project description:To investigate the role of the hypothalamus in complex traits, we generated ATAC-seq, RNA-seq, and Promoter Focused Capture C to gain insight into the gene regulatory arcitecture contacting promoters in an embryonic stem cell dervived model of hypothalamic neuron differentiation

2021-10-01 | GSE152092 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data