Project description:Hepatocellular carcinoma (HCC) is a significant global cancer burden, with raising incidence and lacks a unified prevention strategy due to complex etiologies. By profiling pan-viral serological antibody repertoires using a microbial phage library among 2,647 study subjects, we have identified a unique set of viral antigens with a significantly reduced serological response in HCC patients compared to healthy individuals. Additionally, a higher serological response to these viral antigens links to better clinical outcomes of patients with chronic liver diseases and HCC. These findings are consistent across different populations across sex, ethnicity, and etiology. We have identified a common epitope (CE1) shared among reactive viral antigens from the highly prevalent rhinovirus and enterovirus families. We demonstrate that CE1 induces T-cell activation and HCC cell killing. Our results suggest that past exposures to members of the Enterovirus genus may be advantageous for cancer patients, highlighting the potential for a viral peptide-based HCC vaccine.
Project description:Enterovirus 71 (EV71) belongs to human enterovirus species A of the genus Enterovirus within the family Picornaviridae. We established transformant cells by transfection of mouse cells with genomic DNA from human cells and then detected two EV71-susceptible cell lines. Using microarray with the two cell lines we found that scavenger receptor B2 is a cellular receptor for EV71.
Project description:Enterovirus 71 (EV71), a member of the Enterovirus genus in the Picornaviridae family, was first recognized as a dermotrophic virus that usually cause mild, self-limiting hand-foot-and-mouth disease (HFMD). However, EV71 infection can sometimes induce a variety of severe neurological complications, pulmonary edema and even death. Here, we aimed to provide an overview of proteomics characterization of EV71-infected brain and lung tissues.