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An endogenous retroviral element co-opts an upstream regulatory sequence to achieve somatic expression and mobility

ABSTRACT: An endogenous retroviral element co-opts an upstream regulatory sequence to achieve somatic expression and mobility

PROVIDER: PRJNA1202554 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31821900_1.fastq.gz	Fastqsanger.gz
	SRR31821900_2.fastq.gz	Fastqsanger.gz
	SRR31821901_1.fastq.gz	Fastqsanger.gz
	SRR31821901_2.fastq.gz	Fastqsanger.gz
	SRR31821902_1.fastq.gz	Fastqsanger.gz

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An endogenous retroviral element co-opts an upstream regulatory sequence to achieve somatic expression and mobility

Project description:We characterize somatic retrotransposition in the Drosophila intestine using long-read DNA sequencing. We show that retroelement mobility does not change significantly upon aging and is limited to very few active sub-families of retrotransposons. Importantly, we identify a polymorphic donor locus of an endogenous LTR retroviral element rover, active in the intestinal tissue. We reveal that gut activity of the rover donor copy depends on its genomic environment. Without affecting local gene expression, the copy co-opts its upstream enhancer sequence, rich in transcription factor binding sites, for somatic expression. We further show that escargot, a snail-type transcription factor critical for gut progenitor cell function, can drive transcriptional activity of the active rover copy. These data provide new insights into how locus-specific features allow active retrotransposons to produce functional transcripts and mobilize in a somatic lineage.

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