Project description:With this experiment we aimed do identify eventual genes that are differentially expressed by the fungal pathogen Blumeria graminis triticale when it grows on two different hosts (wheat and triticale) We used to fungal isolates, for each of them we infected wheat and triticale and we extracted RNA (and sequenced) from the infected plant tissue. Three technical replicates for each combinations plant-pathogen were used
Project description:In this study, we used the Affymetrix wheat GeneChip to examine the transcript accumulation in a near-isogenic line pair carrying resistant and susceptible alleles at the wheat Fhb1 locus. The objectives of this study were: (1) to identify the overall response in wheat to F. graminearum infection; (2) to identify key genes involved in FHB resistance/susceptibility pathways in wheat; (3) to compare the transcript profiles of wheat and barley during F. graminearum infection; and (4) to examine the relationship between transcript accumulation, disease severity, fungal biomass and trichothecene accumulation in wheat. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Seungho Cho. The equivalent experiment is TA20 at PLEXdb.]
Project description:Recent attempts to increase endogenous disease resistance of plants by overexpression of anti-fungal transgenes have shown a potential of this method. However, it has also been shown that such improvements are usually small. One of the obvious reasons for this low anti-fungal effect might be the regulation of endogenous genes in parallel. In this project, we will study the effect of anti-fungal transgenes on the endogenous gene expression. Such effects might relate to substantial equivalence which is a biosafety issue of concern to the public. The GeneChip Wheat Genome Array will be used to detect expression of defence response genes and key genes of metabolic pathways. We will use wheat plants transformed with anti-fungal gene of specific effect against a small group of seed transmitted, pathogenic fungi (KP4 against smuts and bunts). Transformed spring wheat line will be challenged by stinking smut (inhibited by KP4). The effect on the endogenous gene expression will be tested for plants grown in the field in collaboration with the USDA Department. This work will contribute to our understanding of plant defence responses in general and may allow improving strategies to strengthen these responses.
Project description:With this experiment we aimed do identify eventual genes that are differentially expressed by the fungal pathogen Blumeria graminis triticale when it grows on two different hosts (wheat and triticale)
Project description:Transcriptional time course analysis of Wheat leaves in response to exposure to the fungal toxin ToxA from Pyrenophora tritici-repentis.
Project description:The aim of this work is to dissect the plant component of the dual-proteome established during the FHB process using the same three wheat cultivars facing the three fungal strains as described in Fabre et al. (2019b). Qualitative and quantitative dissection of the three wheat cultivar proteomes was devoted to identify molecular events that drive the FHB-susceptibility differences. This included, the identification of (i) the generic molecular adjustments taking place during FHB progress, (ii) the cultivar-specific responses and their accommodation with different F. graminearum strains inducing FHB and (iii) the range of wheat proteins that basically discriminate the three wheat cultivars of contrasted susceptibility. The joint analysis of all these data with the fungal protein information was carried out to identify relationship between wheat protein abundance changes and fungal effectors differentially accumulated between the three F. graminearum strains (Fabre et al., 2019b).
Project description:Recent attempts to increase endogenous disease resistance of plants by overexpression of anti-fungal transgenes have shown a potential of this method. However, it has also been shown that such improvements are usually small. One of the obvious reasons for this low anti-fungal effect might be the regulation of endogenous genes in parallel. In this project, we will study the effect of anti-fungal transgenes on the endogenous gene expression. Such effects might relate to substantial equivalence which is a biosafety issue of concern to the public. The GeneChip Wheat Genome Array will be used to detect expression of defence response genes and key genes of metabolic pathways. We will use wheat plants transformed with anti-fungal gene of specific effect against a small group of seed transmitted, pathogenic fungi (KP4 against smuts and bunts). Transformed spring wheat line will be challenged by stinking smut (inhibited by KP4). The effect on the endogenous gene expression will be tested for plants grown in the field in collaboration with the USDA Department. This work will contribute to our understanding of plant defence responses in general and may allow improving strategies to strengthen these responses. Teliospores of pathogenic races T-1, T-5 and T-16 of T. caries provided by a collection in Aberdeen, ID, USA were used for the tests. Seeds of the genetically engineered Swiss spring wheat variety Greina (GrKP4) and the null-segregant control line (Gr0) were coated with spores and Individual plants were scored for bunt symptoms. For microarray analysis only samples inoculated with T1 and T16 were used.