Rattus norvegicus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA120591 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Gene level analysis of lactating day 10-11 16h Sprague-Dawley dams compared against age matched virgins. Lactation is a time of significantly increased energy demands imposed by the suckling young that requires a proportional adjustment in the ability of the lactating dam to absorb nutrients and to synthesize critical biomolecules to meet the dietary needs of both the offspring and the dam. We have shown an increase in the size and hydrophobicity of the bile acid pool during lactation [1], implying an increased absorption and disposition of lipids, sterols, nutrients, and xenobiotics. In order to investigate changes at the level of mRNA, we utilized an exon array and calculated gene level summaries to investigate changes in gene expression in the liver, duodenum, jejunum, and ileum of lactating dams when compared against age-matched virgin controls. Affymetrix Rat Exon 1.0ST arrays were used to determine expression of genes in the livers and small intestines of day 10-11 lactating Sprague Dawley rats and compared against virgins of comparable age.
2010-11-21 | E-GEOD-19175 | biostudies-arrayexpress
Project description:Gene level analysis of lactating day 10-11 16h Sprague-Dawley dams compared against age matched virgins. Lactation is a time of significantly increased energy demands imposed by the suckling young that requires a proportional adjustment in the ability of the lactating dam to absorb nutrients and to synthesize critical biomolecules to meet the dietary needs of both the offspring and the dam. We have shown an increase in the size and hydrophobicity of the bile acid pool during lactation [1], implying an increased absorption and disposition of lipids, sterols, nutrients, and xenobiotics. In order to investigate changes at the level of mRNA, we utilized an exon array and calculated gene level summaries to investigate changes in gene expression in the liver, duodenum, jejunum, and ileum of lactating dams when compared against age-matched virgin controls.
2010-11-21 | GSE19175 | GEO
Project description:Early full-term pregnancy affords lifetime protection against development of breast cancer. Parity-induced protection can be reproduced in a carcinogen-induced rat mammary carcinoma model. The molecular mechanisms of parity-induced protection against carcinogenic stimuli in rat mammary glands have not been fully characterized. In order to gain a better understanding of these molecular mechanisms, we performed gene expression analyses in parous and age-matched virgin (AMV) mammary glands of Lewis rats before and after carcinogen (N-methyl-N-nitrosourea; MNU) treatment. Keywords: other
2005-07-31 | GSE2726 | GEO
Project description:Cross-species hybridization analysis of mammary glands during pregnancy and lactation. Results provide insight into putative conserved molecular mechanisms regulating mammary gland development. This study was performed to identify orthologous transcripts that are differentially co-expressed in the mammary gland at 2 stages of development (pregnancy and lactation) in wild type Sprague-Dawley rats. Key points are examined in a time series of Sprague Dawley rat mammary gland development, secretory activation and lactation. Triplicate rat (three biological replicates) at each time point were used for statistical power totalling 12 individual arrays in this study. Rats were as staged pregnant day 1 the day that post coital plug was observed, and similarly, lactation day 1 was the first day after birth. Whole mammary glands No. 4 (inguinal) were obtained from female rats at stages of development: virgin (adulthood, 14 wks of age), Pregnant (5 and 14 days of pregnancy) and Lactating (day 1 and 12 postpartum). The two-color (Cy5/Cy3) microarray experiment was designed to hybridize samples from each group against a common reference, a pool of RNA from mammary gland of three parous or virgin female rats.
2013-10-30 | E-GEOD-22545 | biostudies-arrayexpress
Project description:Parkinson’s disease (PD) is a progressive degenerative disease. Behaviorally, voice deficits including soft monotone speech appear early and are present in 90% of individuals with PD. However, the pathology, at the level of the nucleus ambiguus, mediating voice deficits is unknown. RNA sequencing was used to examine nucleus ambiguus differential gene expression in male Pink1-/- rats compared to age-matched wildtype controls.
2024-05-01 | GSE213889 | GEO
Project description:Early full-term pregnancy affords lifetime protection against development of breast cancer. Parity-induced protection can be reproduced in a carcinogen-induced rat mammary carcinoma model. The molecular mechanisms of parity-induced protection against carcinogenic stimuli in rat mammary glands have not been fully characterized. In order to gain a better understanding of these molecular mechanisms, we performed gene expression analyses in parous and age-matched virgin (AMV) mammary glands of Lewis rats before and after carcinogen (N-methyl-N-nitrosourea; MNU) treatment.
2008-06-12 | E-GEOD-2726 | biostudies-arrayexpress
Project description:This study was performed to identify transcripts that are differentially expressed in the mammary gland at 4 stages of developmental (virgin, pregnant, lactating and involution) in wild type C57BL/6J mice. Experiment Overall Design: Whole mammary glands No4 (inguinal) were obtained from female mice at 4 stages of development: Virgin (puberty, 6 wks of age), Pregnant (14 days of pregnancy), Lactating (day 10 postpartum) and Involution (4 days post weaning of pups). Three biological replicates were obtained from Virgin females and two biological replicates for the three other stages. mice
2007-11-13 | E-GEOD-5831 | biostudies-arrayexpress
Project description:Purpose: To characterize the transcriptional responses to TRPC5 inhibition in vivo, we compared gene expression profiles (RNASeq) in isolated glomeruli from wild type (WT) rats, AC1903-treated AT1R transgenic (Tg) rats in the advanced cohort and vehicle-treated, age-matched AT1R controls. Methods: Glomerular mRNA profiles of WT rats, AC1903-treated AT1R transgenic (Tg) rats in the advanced cohort and vehicle-treated, age-matched AT1R controls were generated by deep sequencing using Illumina. Sequenced reads were mapped by STAR and quanitifed using RSEM. Differential expression analysis and Gene Ontology term enrichment analysis was performed. Results: We identified 541 differentially expressed genes in AT1R Tg rats compared to WT controls. Interestingly, Gene Ontology (GO) term enrichment analysis revealed ROS-related and ion (cation) channel and transporter activity gene signatures, in line with the hypothesis that Rac1-mediated ROS generation and TRPC5 Ca2+ (cation) mediated signaling lead to disease progression. After treatment with AC1903, 42 genes were differentially expressed in AC1903-treated versus vehicle-treated AT1R Tg rats. This smaller number of genes is consistent with the notion that AC1903 targets a specific pathway triggered by TRPC5 activity to confer its therapeutic advantage. GO term enrichment analysis revealed cell adhesion and integrin signaling gene sets, consistent with our observations in vivo that AC1903 protects podocytes from attenuations, detachment and loss. Conclusions: In short, the in vivo gene expression profiles support a Rac1-TRPC5 disease-promoting pathway in proteinuric kidney disease that is reversed by treatment with AC1903.
2017-10-01 | GSE103020 | GEO
Project description:Chronic hypertension during pregnancy affects both mother and infant but its pathological mechanisms are unclear. Due to ethical limitations human data is available in term tissue only- so deeper understanding of the vascular changes during early pregnancy are unknown. This study utilises a known rat model of chronic hypertension to study changes in gene expression at an early gestational time point. After 6 days of pregnancy animals were sacrificed and main uterine arteries harvested for RNA-Seq (or age-matched virgin rats as controls).
2021-03-15 | E-MTAB-10212 | biostudies-arrayexpress
Project description:The lactating mammary glands in ruminants play a pivotal economic role in agriculture and food nutrition. The need for an improved 3D in vitro model to study its lactating biology highlights the importance of ongoing research in this field. Overcoming these challenges represents a crucial area of research, with the development of protocols for livestock lactating organoids being a significant goal for the future. In this study, we utilized virgin dairy goats as a model and successfully established a mammary organoid system. These organoids, cultured within an extracellular matrix (ECM) gel, maintained a bilayer structure that closely resembled the original mammary tissue architecture. Importantly, the expression patterns of genes related to fatty acid synthesis and milk proteins in lactating organoids closely mirrored those observed in lactating mammary tissues. Proteomic analysis further confirmed that major milk proteins were highly produced in the lactating organoids. In summary, this study successfully developed a mammary organoid culture system that holds significant promise as a novel platform for cell-based milk production.
2025-05-06 | PXD050745 | Pride