Project description:Purpose: The goal of this study is to compare endothelial small RNA transcriptome to identify the target of OASL under basal or stimulated conditions by utilizing miRNA-seq. Methods: Endothelial miRNA profilies of siCTL or siOASL transfected HUVECs were generated by illumina sequencing method, in duplicate. After sequencing, the raw sequence reads are filtered based on quality. The adapter sequences are also trimmed off the raw sequence reads. rRNA removed reads are sequentially aligned to reference genome (GRCh38) and miRNA prediction is performed by miRDeep2. Results: We identified known miRNA in species (miRDeep2) in the HUVECs transfected with siCTL or siOASL. The expression profile of mature miRNA is used to analyze differentially expressed miRNA(DE miRNA). Conclusions: Our study represents the first analysis of endothelial miRNA profiles affected by OASL knockdown with biologic replicates.
Project description:<p><em>Ulmus parvifolia</em> (<em>U. parvifolia</em>) was traditionally used in the treatment of some diseases such as inflammation, diarrhea and fevers. With the wide application of molecular biotechnology in plant development and utilization, the pharmaceutical value and chemical composition of the bark and leaves of <em>U. parvifolia</em> had been studied. However, metabolic signatures of seeds have not been studied. Seeds and bark of <em>U. parvifolia</em> were collected at the seeds ripening stage and metabolite profiling was performed through the untargeted metabolomics approach. A total of 2,578 and 2,207 metabolites were identified in seeds and bark, respectively. In particular, 574 differential metabolites were identified from the two parts of <em>U. parvifolia</em>, including flavonoids, terpene glycosides, triterpenoids and sesquiterpenoids. There are some bioactive compounds with antioxidant, anti-inflammatory and anti-cancer activities in these metabolites. More metabolites belonged to flavonoids and sesquiterpenoids were up-regulated in seeds than that in bark. There were more varieties of terpene glycosides and triterpenoids in bark than seeds. The pathway enrichment was performed, while flavonoid biosynthesis and flavone and flavonol biosynthesis were worthy of attention. Metabolic signatures of <em>U. parvifolia</em> seeds and bark were analyzed by untargeted metabolomics approach. Metabolite profiling, characteristics of differential metabolites and pathway enrichment were performed to evaluate the pharmaceutical value of seeds and bark. This study provided a scientific basis for the complementary use of the two different parts of <em>U. parvifolia</em> as a Chinese medicinal material.</p>
Project description:A cDNA library was constructed by Novogene (CA, USA) using a Small RNA Sample Pre Kit, and Illumina sequencing was conducted according to company workflow, using 20 million reads. Raw data were filtered for quality as determined by reads with a quality score > 5, reads containing N < 10%, no 5' primer contaminants, and reads with a 3' primer and insert tag. The 3' primer sequence was trimmed and reads with a poly A/T/G/C were removed
Project description:Whole exome sequencing of 5 HCLc tumor-germline pairs. Genomic DNA from HCLc tumor cells and T-cells for germline was used. Whole exome enrichment was performed with either Agilent SureSelect (50Mb, samples S3G/T, S5G/T, S9G/T) or Roche Nimblegen (44.1Mb, samples S4G/T and S6G/T). The resulting exome libraries were sequenced on the Illumina HiSeq platform with paired-end 100bp reads to an average depth of 120-134x. Bam files were generated using NovoalignMPI (v3.0) to align the raw fastq files to the reference genome sequence (hg19) and picard tools (v1.34) to flag duplicate reads (optical or pcr), unmapped reads, reads mapping to more than one location, and reads failing vendor QC.