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Modeling human gastrulation and secondary yolk sac formation with BMP4 signaling center

ABSTRACT: Modeling human gastrulation and secondary yolk sac formation with BMP4 signaling center

PROVIDER: PRJNA1207972 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31929588_1.fastq.gz	Fastqsanger.gz
	SRR31929588_2.fastq.gz	Fastqsanger.gz
	SRR31929589_1.fastq.gz	Fastqsanger.gz
	SRR31929589_2.fastq.gz	Fastqsanger.gz
	SRR31929590_1.fastq.gz	Fastqsanger.gz

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Modeling human gastrulation and secondary yolk sac formation with BMP4 signaling center

Project description:Purpose: Characterization of cell types in BMP4 induced human gastruloids. Methods: BMP4 induced human gastruloids were harvested on days 2, 4, 6, 8, 10, and 15, and then dissociated into single cells. Libraries were constructed with a Chromium Single Cell 3′v3 Reagent Kit (10x Genomics) according to the manufacturer’s protocol for 10000 cell recovery.Sequencing was performed with Illumina Novaseq 6000 according to the manufacturer’s instructions (Illumina). Results: Definiitve endoderm, yolk sac endoderm, hematopoiesis cell fates were detected in BMP4 induced human gastruloids. Conclusions: BMP4 induced the formation of human gastruloids with a yolk sac-like cavity structure.

2025-01-20 | GSE286120 | GEO

Expression data from Tnrc6a (GW182) mutant yolk sac

2011-09-01 | GSE30244 | GEO

Expression data from Tnrc6a (GW182) mutant yolk sac

Project description:GW182 (Tnrc6a) is a key component of RISC (miRNA-Induced Silencing Complex) that plays a critical role in miRNA-mediated gene silencing. Here, we show that GW182 is expressed in the yolk sac endoderm, and that gene-trap disruption of GW182 leads to growth arrest of yolk sac endoderm, impaired hematopoiesis and embryonic lethality. To investigate roles of GW182 in the yolk sac endoderm, we assessed changes in mRNA expression in the yolk sac of E9.5 GW182gt/gt embryos using microarrays (Affymetrix). Yolk sac of wild type littermates and GW182gt/gt embryos at E9.5 was collected for total RNA isolation using Trizol (Invitrogen). RNAs were purified according to the manufacturer’s protocol before subjected to Mouse Gene 1.0 ST Whole Genome Array (Affymetrix) for mRNA expression profiling. Experiments were performed in triplicate. Differentially expressed mRNAs were identified using a two-sample t-test (P<0.05 considered significant).

2011-08-31 | E-GEOD-30244 | biostudies-arrayexpress

The role of the yolk sac in human fetal development and identification of a hepatocyte-like cell in the human yolk sac

Project description:The human yolk sac (YS) is an extra-embryonic tissue critical for early prenatal life development. It is the first site of haematopoiesis where progenitors differentiate from endoderm within blood islands of the yolk sac contributing initially to primitive erythropoiesis and in subsequent waves to erythro-myeloid and lymphoid differentiation.

2023-03-01 | E-MTAB-10552 | biostudies-arrayexpress

The role of the yolk sac in human fetal development and identification of a hepatocyte-like cell in the human yolk sac (SS2)

2023-03-01 | E-MTAB-10888 | biostudies-arrayexpress

Modeling Post-Implantation Human Development to Yolk Sac Blood Emergence

Project description:Implantation of the human embryo commences a critical developmental stage that comprises profound events including axis formation, gastrulation, and the emergence of yolk sac hematopoietic system. Our mechanistic knowledge of this window of human life remains limited due to restricted access to in vivo samples for both technical and ethical reasons. Stem cell models of human embryo have emerged to help unlock the mysteries of this stage. Here, we present a model of early post-implantation human embryogenesis (embryoid) with an extra-embryonic endoderm and mesoderm niche to support early hematopoiesis. This human embryo model is genetically inducible, and its unanticipated self-organization results in the development of the amniotic cavity, formation of bilaminar disc embryonic morphology, an anterior hypoblast pole and posterior domain. The extra-embryonic layer of iDiscoid presents multilineage yolk sac tissue morphogenesis with extra-embryonic mesoderm and lacks trophoblast. Its development captures distinct waves of yolk sac hematopoiesis including the emergence of erythroid, myeloid and lymphoid cells . iDiscoid offers an easy-to-use, high-throughput, reproducible, and scalable platform to probe multifaceted aspects of human development and blood formation at early post-implantation stage. It will provide a tractable human-based model for drug testing, disease modeling, and a unique cell source for regenerative medicine.

2023-11-10 | GSE247111 | GEO

Mouse complete embryoid development within a yolk sac recapitulates gastrulation, neurulation and early organogenesis

Project description:Mouse complete embryoid development within a yolk sac recapitulates gastrulation, neurulation and early organogenesis

| PRJNA862479 | ENA

Mouse complete embryoid development within a yolk sac recapitulates gastrulation, neurulation and early organogenesis

Project description:Embryo-like structures generated from stem cells can achieve varying developmental milestones, but none have been shown to progress through gastrulation, neurulation, and organogenesis. Here, we show that "ETiX" mouse embryoids, assembled from embryonic stem cells, trophoblast stem cells and inducible extraembryonic endoderm stem cells, can develop into gastrulating embryoids, and beyond to generate neurulating embryoids, which generate the progenitors needed to create the entire organism. The head-folds of ETiX neurulating embryoids show anterior expression of Otx2, defining forebrain and midbrain regions that resemble those of the natural mouse embryo. Neurulating embryoids also develop beating heart-like structures, trunks comprising a neural tube and somites, tail buds containing neuromesodermal progenitors and primordial germ cells, and gut tubes derived from definitive endoderm. Notably, neurulating embryoids also develop a yolk sac with blood islands. Overall, ETiX neurulating embryoid formation strongly resembles natural embryogenesis, advancing embryo-like development further than any other stem-cell derived model and within extra-embryonic membranes.

2022-08-12 | GSE209792 | GEO

Human fetal yolk sac scRNA-seq data (sample ID: F158 for Haniffa Lab; 16099 for HDBR)

Project description:Investigating the blood, immune and stromal cells present in a human fetal embryo in a world first single cell transcriptomic atlas. The embryo was dissected into 12 coronal sections, yolk sac, and yolk sac stalk. Live single cells sorted, with cell suspension then undergoing 10x chromium 5 prime scRNA-seq. This accession contains the yolk sac and yolk sac stalk data from this embryo. A matched accession contains the coronal section data. Lane "WS_wEMB12142156" (from yolk sac) was excluded from downstream analysis due to low fraction reads in cells post-CellRanger QC. Termination procedure for this embryo was medical. The F158_[features...barcodes...matrix].[tsv...mtx].gz files attached to this accession represent raw count data from all the 10x lanes in this accession combined, and as output from CellRanger filtered matrices (CellRanger version 6.0.1 using human reference genome GRCh38-2020-A). One set of count matrices relates to the yolk sac data, and one set of count matrices relates to the yolk sac stalk data.

2022-12-07 | E-MTAB-11673 | biostudies-arrayexpress

Gene expression profiling of rudhira null embryo and yolk sac at 9.5 dpc

Project description:Rudhira is essential for mouse developmental angiogenesis and tissue morphogenesis. Embryos lacking endothelial rudhira die at mid-gestation with vascular patterning defects. Rudhira mutant yolk sac endothelial cells show slow and random migration. So to identify key signaling pathways perturbed in the absence of rudhira, we undertook whole transcriptome based analysis of gene expression in rudhira null yolk sac and embryo. Transcriptome analysis shows that key mediators of angiogenesis, cell adhesion, migration and extracellular matrix degradation as well as several components of the TGFÎ² pathway are perturbed in rudhira null mutant yolk sacs at 9.5 dpc. We used two embryo samples ( 2E- wild type; 5E- rudhira mutant). Repetition was done on each sample. We used 4 yolk sac samples (3Y,4Y- wild type; 7Y,8Y-rudhira mutant) for the analysis. Repetition was done on each sample.

2015-12-31 | E-GEOD-69204 | biostudies-arrayexpress

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