Project description:IFN-Ⅰ plays an important role in the progression of LGG. We used a set of genes from MsigDB associated with interferon alpha responses, bioinformatic screening based on public databases, and validation of internal and external data to obtain relevant biomarkers. Immunohistochemical results of clinical samples showed that the higher the grade of glioma, the higher the expression of RIPK2 gene. Subsequently, U118 knockdown RIPK2 cell line was constructed, and the knockdown group could inhibit the proliferation of tumor cells and promote apoptosis. RNA-seq sequencing results showed that, compared with the control group, GO enrichment analysis showed that the differential genes were mainly concentrated in bioregulatory and molecular function related pathways such as ion gated channel activity and cell adhesion, and KEGG enrichment analysis showed that, Genes were mainly enriched in inflammatory pathways such as NF-kappa B signaling pathway, TNF signaling pathway and IL17 signaling pathway.
Project description:RIPK4 but not the related kinases RIPK1, RIPK2, and RIPK3 caused similar transcriptional changes to Wnt3a. PA1 cells were transfected by 8ug RIPK1, RIPK2, RIPK3, or RIPK4 for 48h, RNA were extracted and sequenced.
Project description:Analysis of DDX20 knockdown - hepatocellular carcinoma cells. The expression levels of genes driven by NF-kB and related with carcinogenesis, were significantly enhanced in DDX20-knockdown cells. One condition experiment, HepG2 vs. HepG2-DDX20 knockdown cells
Project description:To test the effect of Spag4 knockdown, we compared knockdown and control cells subjected to 48h 2uM camptothecin treatment or vehicle.
Project description:Nuclear receptor subfamily 1, group D, member 1 (Nr1d1) (also known as Rev-erb alpha) has been linked to circadian rhythm regulation, mood-related behavior, and disorders associated with social deficits. Recent work from our laboratory found striking decreases in Nr1d1 in nucleus accumbens (NAc) in the maternal condition and indirect evidence that Nr1d1 was interacting with numerous addiction and reward-related genes to modulate social reward. In this study, we applied our insights from the maternal state to non-parental adult mice to determine whether decreases in Nr1d1 expression in NAc via adeno-associated viral (AAV) vectors and short hairpin RNA (shRNA)-mediated gene knockdown were sufficient to modulate social reward and mood-related behaviors. We also used microarray analysis of to identify gene expression alterations induced by the lowering of Nr1d1 expression. We used microarrays to evalute the effects of knockdown of mRNA for Nr1d1 in nuclues accumbens on gene expression.
Project description:Analysis of DDX20 knockdown - hepatocellular carcinoma cells. The expression levels of genes driven by NF-kB and related with carcinogenesis, were significantly enhanced in DDX20-knockdown cells.