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ABSTRACT: Transcriptomics of 84K poplar under salt stress

PROVIDER: PRJNA1210839 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32004500_1.fastq.gz	Fastqsanger.gz
	SRR32004500_2.fastq.gz	Fastqsanger.gz
	SRR32004489_1.fastq.gz	Fastqsanger.gz
	SRR32004489_2.fastq.gz	Fastqsanger.gz
	SRR32004490_1.fastq.gz	Fastqsanger.gz

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Project description:Transcriptomics of Poplar under salt stress

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Genes expression during stem growth of Populus alba x Populus tremula var. glandulosa

Project description:To obtain genes expression in different parts of 84k poplar stems, transcriptome sequencing was performed using Illumina Novaseq 6000 second-generation sequencing platform from Shanghai BIOZERON Co. Ltd (www.biozeron.com). Selecte three stem segments of plants REPEAT 1, 2 and 3 with good and similar growth to use: 2nd-3rd internodes (poplar stem top: PST1, PST2, PST3); 9th-10th internodes (poplar stem middle: PSM1, PSM2, PSM3); 14th-15th internodes (poplar stem bottom: PSB1, PSB2, PSB3). [Or the three repeating organisms are also called poplar A, B, C. From top to bottom, the three parts of the stem are also called stem 1, 2, 3.]

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Project description:Functional analysis of PagERF021 gene in salt stress tolerance in 84k poplar

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Analysis of Expression Profile of Over-expressed Brassinolide Synthase DET2 in Poplar

Project description:We overexpressed the brassinolide synthase DET2 in 84K poplar and obtained an overexpression material.It was found that the brassinolide content of the overexpression material increased significantly.And it was found that the plant height, diameter, and number of internodes of the overexpression material increased significantly.However,the mechanism of this phenotype is unknown.We collected two-month-old DET2 overexpression material and 84K wild-type material in three parts, apex,cambium,and xylem,and conducted high-throughput sequencing.The sequencing results were mined, and the difference genes that were significantly changed in the DET2 overexpression material and the 84K wild-type material were found.Find out how the increase in brassinolide content promotes the development of wood.

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Project description:Transcriptome data of poplar under salt stress

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Project description:Populus euphratica is a medium-sized deciduous tree naturally grown in high saline condition, however, the molecular response of the poplar to salinity at global genome level maintain to be elucidated. We used Affymetrix poplar genome microarrays to investigate the full transcript expression exposed to different salt intensities and identified significantly changed transcripts within the 24 hours after exposed to salt stress.

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Project description:Root transcriptomes of poplar hybrid 84K (P. alba x P. tremula var. glandulosa) under drought and salt stress

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Project description:84K poplar transcriptome

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Salt treatment of PtSnRK2.7 overexpressor

Project description:Subfamily 2 of SNF1-related protein kinase (SnRK2) plays important roles in plant abiotic stress responses as a global positive regulator of abscisic acid signaling. In the genome of the model tree Populus trichocarpa, 12 SnRK2 genes have been identified, and some are upregulated by abiotic stresses. In this study, we heterologously overexpressed the PtSnRK2 genes in Arabidopsis thaliana and found that overexpression of PtSnRK2.5 and PtSnRK2.7 genes enhanced stress tolerance. In the PtSnRK2.5 and PtSnRK2.7 overexpressors, chlorophyll content and root elongation were maintained under salt stress conditions, leading to higher survival rates under salt stress compared with those in the wild type. Transcriptomic analysis revealed that PtSnRK2.7 overexpression affected stress-related metabolic genes, including lipid metabolism and flavonoid metabolism, even under normal growth conditions. However, the stress response genes reported to be upregulated in Arabidopsis SRK2C/SnRK2.6 and wheat SnRK2.8 overexpressors were not changed by PtSnRK2.7 overexpression. Instead, PtSnRK2.7 overexpression widely and largely influenced the transcriptome in response to salt stress; genes related to transport activity, including anion transport-related genes, were characteristically upregulated, and a variety of metabolic genes were specifically downregulated. We also found that the salt stress response genes were greatly upregulated in the PtSnRK2.7 overexpressor. Taken together, poplar subclass 2 PtSnRK2 genes can modulate salt stress tolerance in Arabidopsis, through the activation of cellular signaling pathways in a different manner from that by herbal subclass 2 SnRK2 genes.

2016-08-01 | GSE79997 | GEO

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Project description:We performed that comprehensive identification of genes responsible for stress tolerance by analyzing the whole-genome expression profiles of poplar (Populus alba × P. glandulosa) leaves exposed to drought and salt stresses. Examination at the molecular level how this tree species responds to drought and salt stresses by regulating the expression of genes involved in signal transduction, transcriptional regulation, and stress responses.

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