Project description:Oak galls form when gall wasps lay their eggs into part of the tree; in some galls, this attachment point to the host consists of only a few cells. The gall itself comprises entirely of host tissue; however, the initiation, development, and physical appearance are controlled by the inducer. This raises the intriguing question of the molecular mechanisms underlying gall formation, by which one or a small number of cells are reprogrammed and commit to a novel developmental path. Gall wasps undergo two generations each year, and the galls formed by these two generations exhibit markedly different appearances. We sequenced the transcriptomes of both the sexual and asexual generations of Neuropterus quercusbaccarum and Neuroterus numismalis. The transcriptomes of the generations that occur at the same time of year are more similar to each other than they are to the opposite generation of their respective species.
2024-06-18 | GSE244168 | GEO
Project description:whole-genome sequencing of three amphitriploid generations and their parents
Project description:We used RNA sequencing to examine the transcriptomes of male and female heads from experimentally-evolved D. melanogaster populations after 117 generations of mating system manipulation in order to examine the pattern of evolution in sex-biased genes. Examined head transcriptomes of 3 monogamous populations and 3 polygamous populations, both males and females, for 12 total samples.
Project description:We used RNA sequencing to examine the transcriptomes of male and female heads from experimentally-evolved D. melanogaster populations after 117 generations of mating system manipulation in order to examine the pattern of evolution in sex-biased genes.
Project description:In traditional models of Drosophila flies and C. Elegans worms have shown epigenetic transgenerational inheritance has persisted and remained stable for hundreds of generations. Mammalian studies, however, have primarily investigated up to three or four generations. Environmental exposures to toxicants or other stressors have been linked to having a role in epigenetic alterations and epigenetic transgenerational inheritance. Changes within the epigenome have the potential to change gene expression and genetic processes, as well as potentially alter phenotypes and evolution in all organisms. In this study, an outcrossed mammalian colony of outbred rats was bred for twenty three successive generations and assessed for epigenetic alterations and incidence of pathological abnormalities. Sperm from male rats were analyzed for DNA methylation and pathology abnormalities in the female and male rats in successive generations. Observations showed an increase in disease incidence within the ten generations (F13) and twenty generations (F23) compared to the control lineage. Our results indicate that ten generations of transgenerational exposure led to an increase in epigenetic alterations and a higher incidence of pathological abnormalities. Ancestrally exposed rats to vinclozolin showed significant parturition abnormalities in both the maternal and paternal lineages after 16 generations. This included maternal deaths during labor and stillbirths. Pathological assessments revealed abnormalities across multiple tissue types and an increased incidence of disease. This suggests the physiological consequences of the generational stability of epigenetic inheritance. Observations establish the generational stability of epigenetic inheritance over twenty generations in a mammalian model system, however, new pathology in later generations involving parturition abnormalities were also observed.