Project description:In order to explore the mechanism of ZYHT Recipe on gastric cancer, we performed a RNA-seq to explore the transcriptome profiling of gastric cancer cells treatment with or without ZYHT ,including three biological replicates per group.
Project description:The Wenshenyang recipe (WSYR) has the effect of treating infertility, but the mechanisms underlying this activity have not been fully elucidated. In this study, network pharmacology and RNA sequencing were combined, with database-based “dry” experiments and transcriptome analysis-based “wet” experiments used conjointly to analyse the mechanism of WSYR in the treatment of infertility. In the dry analysis, 43 active compounds in WSYR and 44 therapeutic targets were obtained through a database search, 15 infertility pathways were significantly enriched, and key targets, such as ESR1, TP53, AKT1, IL-6, and IL-10 were identified. In the wet experimental analysis, HK-2 cells were treated with the three herbs of WSYR. Using the transcriptome sequencing platform, the expression level changes in 412 genes from 15 infertility pathways were observed, and 92 DEGs were finally obtained. Additionally, key targets, such as ESR2, STAT1, STAT3, and IL6, were identified. RT-qPCR experiments further verified that WSYR played an anti-inflammatory role by upregulating IL-4 and IL-10. By building the molecular docking model of ESR1, ESR2 and screening compounds, it was further found that xanthogalenol in Drynaria fortunei (Kunze) J.Sm. (Gusuibu), arachidonate in Cistanche deserticola Y.C.Ma (Roucongrong), and anhydroicaritin in Epimedium brevicornu Maxim. (Yinyanghuo) have good affinity for estrogen receptors. These findings provide evidence that WSYR exerts an estrogen-regulating role. By integrating the results of dry and wet experiments, it was found that the WSYR treats infertility by regulating hormone levels and inflammatory responses.
Project description:MicroRNAs (miRNAs) have emerged as potential therapeutic targets for non-alcoholic fatty liver disease/non-alcoholic steatohepatitis (NAFLD/NASH). Traditional Chineses Medicine (TCM) plays an important role in the prevention or treatment of NAFLD/NASH. However, miRNA targets of TCM against NASH still remain largely unknown. Here, we showed that Yiqi-Bushen-Tiaozhi (YBT) recipe effectively attenuated diet-induced NASH in C57BL/6 mice. To identify the miRNA targets of YBT and understand the potential underlying mechanisms, we performed network pharmacology using miRNA and mRNA deep sequencing data combined with Ingenuity Pathway Analysis (IPA). Mmu-let-7a-5p, mmu-let-7b-5p, mmu-let-7g-3p and mmu-miR-106b-3p were screened as the main targets of YBT. Our results suggested that YBT might alleviate NASH by regulating the expression of these miRNAs that potentially modulate inflammation/immunity and oxidative stress. This study provides useful information for guiding future studies on the mechanism of YBT against NASH by regulating miRNAs.