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Mettl3 regulates lens development by promoting the differentiation processes of secondary fiber cells [m6a-seq]

ABSTRACT: Mettl3 regulates lens development by promoting the differentiation processes of secondary fiber cells [m6a-seq]

PROVIDER: PRJNA1213885 | ENA |

REPOSITORIES: ENA

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Mettl3 regulates lens development by promoting the differentiation processes of secondary fiber cells [m6a-seq]

Project description:Lens development requires tight regulation of cell proliferation and differentiation processes, the disruption of which leads to congenital cataract formation. N6-methyladenosine (m6A) is the most prevalent internal mRNA modification and has been shown to play important roles in regulating the development, physiology and pathology of various organs and tissues. However, the function of m6A during lens development remains unknown. In this study, we used lens-specific Mettl3 conditional knockout mice as a model to study the function of Mettl3 during lens development. Our study revealed that Mettl3 plays essential role in lens development by promoting the differentiation processes of secondary fiber cells.

2025-07-23 | GSE287639 | GEO

Mettl3 regulates lens development by promoting the differentiation processes of secondary fiber cells [RNA-seq]

Project description:Lens development requires tight regulation of cell proliferation and differentiation processes, the disruption of which leads to congenital cataract formation. N6-methyladenosine (m6A) is the most prevalent internal mRNA internal modification and has been shown to play important roles in regulating the development, physiology and pathology of various organs and tissues. However, the function of m6A during lens development remains unknown. In this study, we used lens-specific Mettl3 conditional knockout mice as a model to study the function of Mettl3 during lens development. Our study revealed that Mettl3 plays essential role in lens development by promoting the differentiation processes of secondary fiber cells.

2025-07-23 | GSE287640 | GEO

Mettl3 regulates lens development by promoting the differentiation processes of secondary fiber cells [RNA-seq]

Project description:Mettl3 regulates lens development by promoting the differentiation processes of secondary fiber cells [RNA-seq]

| PRJNA1213886 | ENA

Lens Epithelial Cells and Lens Cortical Fiber Cells

Project description:Total RNA was isolated from three separate populations of human lens epithelial cells and three matching populations of lens cortical fiber cells. All samples were analyzed on separate microarrays. Keywords: repeat sample

2005-02-15 | GSE2256 | GEO

Chromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and their denucleation [lens tissue]

Project description:Genome-wide approach to identify the cell-autonomous role of Brg1 in lens fiber cell terminal differentiation. To examine roles of Brg1 in mouse lens development, a dnBrg1 transgenic construct was expressed using the lens-specific alphaA-crystallin promoter in postmitotic lens fiber cells. Morphological studies revealed abnormal lens fiber cell differentiation in transgenic lenses resulting in cataract. Electron microscopic studies showed abnormal lens suture formation and incomplete karyolysis (denucleation) of lens fiber cells. To identify genes regulated by Brg1, RNA expression profiling was performed in E15.5 embryonic wild type and dnBrg1 transgenic lenses. In addition, comparisons between differentially expressed genes in dnBrg1 transgenic, Pax6 heterozygous, and Hsf4 homozygous lenses identified multiple genes co-regulated by Brg1, Hsf4 and Pax6. Among them DNase IIbeta, a key enzyme required for lens fiber cell denucleation, was found downregulated in each of the Pax6, Brg1 and Hsf4 model systems. Lens-specific deletion of Brg1 using conditional gene targeting demonstrated that Brg1 was required for lens fiber cell differentiation and indirectly for retinal development but was not essential for lens lineage formation. Wild type and dnBrg1 transgenic lenses, 4 biological replicates each

2011-01-01 | E-GEOD-22322 | biostudies-arrayexpress

Transcription profiling of human lens epithelial cells and lens cortical fiber cells

2007-08-25 | E-GEOD-2256 | biostudies-arrayexpress

Chromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and their denucleation [lens tissue]

2011-01-01 | GSE22322 | GEO

Nuclear Organization of Lens Epithelium and Fiber Cells in Newborn Lens

Project description:Cellular differentiation is marked by temporally and spatially coordinated gene expression regulated at multiple levels within the nucleus. Sequence-specific DNA-binding transcription factor CTCF EDIT. Topologically associated domains (TADs). Using Hi-C, we investigated changes in chromatin organization between newborn (P0.5) mouse lens fiber and epithelium and compared them to embryonic stem (ES) cells. Compartments A and B. Using ChIP-seq, we determined localization of CTCF in both lens tissues Formation of lens-specific TADs is demonstrated via comparative studies of chromatin at Pax6, Prox1, Gata3, Hsf4, and crystallin loci (to be updated) between lens and ES cell nuclei. Our study has generated the first data on nuclear organization in lens epithelium and lens fibers and directly compared these data with ES cells.

2023-10-16 | GSE243851 | GEO

Chromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and their denucleation

Project description:This SuperSeries is composed of the following subset Series: GSE22322: Chromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and their denucleation [lens tissue] GSE25168: Chromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and their denucleation [eyeball tissue] Refer to individual Series

2011-01-01 | E-GEOD-25169 | biostudies-arrayexpress

Chromatin Remodeling Enzyme Smarca5/Snf2h Regulates Cell Cycle Exit, Differentiation of the Lens Epithelium, and Denucleation of Lens Fiber Cells

Project description:Genome-wide approach to identify the cell-autonomous role of Snf2h in lens fiber cell terminal differentiation. Differential gene expression was analyzed in Snf2h lens-conditional knockout and wildtype newborn mouse eyeballs, with subsequent comparison of this data with the Brg1 lens-conditional knockout mouse eyes expression data (GSE25168). Four biological replicate experiments were performed.

2012-10-18 | E-GEOD-41608 | biostudies-arrayexpress

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