Project description:We have characterized miRNAs associated with equine seminal exosomes, and identified seminal exosomes eca-mir-128 to be specifically downregulated during equine arteritis virus long-term persistent infection in the reproductive tract of the stallion
2018-02-12 | GSE108180 | GEO
Project description:Equine Intestinal Microbiome in Hypersensitivity Disorders
| PRJNA548852 | ENA
Project description:MICROBIAL ECOLOGY OF THE EQUINE REPRODUCTIVE TRACT
Project description:Identify differentially expressed microRNAs in mild and severe equine distal interphalangeal joint osteoarthritis plasma and synovial fluid samples Determine the effects of selected osteoarthritis-related miRNAs on equine chondrocytes in monolayer culture through the application of miRNA agomirs and antagomirs
Project description:Reproductive disorders are a concern in the pig industry. Successful gestation processes are closely related to a suitable endometrial microenvironment, and the physiological mechanisms leading to failed pregnancy during the peri-implantation period remain unclear. We constructed single-cell transcriptome profiles of peri-implantation embryo loss and successful gestation endometrial tissues, and identified 22 cell subpopulations, with epithelial and stromal cells being the predominant endometrial cell types. The two tissues showed marked differences in cell type composition, especially among epithelial cell subpopulations.Overall, the present study revealed the molecular features of endometrial cell transcription in embryo-lost endometrial tissues, providing deeper insights into the endometrial microenvironment of reproductive disorders, which may inform the etiological, diagnostic, and therapeutic studies of reproductive disorders.
Project description:Real- time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a technique that allows for the quantification of mRNA transcripts present within a tissue of interest. To ensure that quantified amounts of mRNA determined by RT qPCR is due to biological differences rather than a product of variation in testing protocol, results need to be normalized. Normalization has historically relied on the use of reference genes, or genes whose transcript expression does not differ in the tissue of interest independent of the experimental condition. In the field of equine reproductive studies, ACTB and GAPDH have been the most widely used reference genes for normalization of RT-qPCR results. However, recent studies have demonstrated that these genes may have drastically varied expression levels in different tissues and in different physiological states. Our study was aimed at examining different putative reference genes (historic reference genes as well as genes identified by RNA-seq to be stable across different sample types) in equine corpus luteum samples at day 11 and day 13 in pregnant and non-pregnant animals. Stability of genetic expression was evaluated via three stability software analyses (GeNorm, NormFinder and BestKeeper). We hypothesized that the most commonly used historic reference genes (ACTB, GAPDH and B2M) would be the most stably expressed genes in equine corpus luteum samples. COX4I1 and SRP14 were both found to be within the top three most stable genes of all samples for all methods. When assessing the least stably expressed genes, the historic reference genes were frequently identified across the three softwares. Exploration of putative reference genes should be considered when investigating dynamic endocrine organs such as those used in reproductive studies. RT-qPCR studies evaluated with historic genes should be interpreted cautiously.
