Escherichia coli Nissle 1917
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ABSTRACT:
PROVIDER: PRJNA1216379 | ENA |
REPOSITORIES: ENA
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Project description:The probiotic strain Escherichia coli Nissle 1917 (EcN), a potential member of tumor-targeting bacteria, shows great promise for cancer treatment. By leveraging engineered EcN, we can design a bacteria-assisted, tumor-targeted therapy for the biosynthesis and targeted delivery of small-molecule anticancer agents. In this study, we aimed to use EcN as a base for synthesizing Romidepsin (FK228), an FDA-approved drug originally made by Chromobacterium violaceum No. 96. Through gene cluster reconstruction, promoter optimization, and genome modification, we created FK228-producing strains to boost anticancer efficacy. The engineered strain achieved a maximum in vitro yield of 1.5 mg/L. In 4T1 tumor-bearing BALB/c mouse xenograft models , six recombinant strains outperformed the wild-type EcN. Proteome showed that inflammatory response induced by EcN combined with intratumoral FK228 production improved treatment results. Also, targeted synthesis reduced FK228's cardiotoxicity and mortality. Engineered EcN enables drug biosynthesis and precise delivery, offering powerful anticancer activity.
2026-02-14 | PXD073783 | Pride
Project description:The Escherichia coli strain Nissle 1917 (EcN) is used as a probiotic for the treatment of certain gastrointestinal diseases in several European and non-European countries. In vitro studies showed EcN to efficiently inhibit the production of Shiga toxin (Stx) by Stx producing E. coli (STEC) such as Enterohemorrhagic E. coli (EHEC). The occurrence of the latest EHEC serotype (O104:H4) responsible for the great outbreak in 2011 in Germany was due to the infection of an enteroaggregative E. coli by a Stx 2-encoding lambdoid phage turning this E. coli into a lysogenic and subsequently into a Stx producing strain. Since EHEC infected persons are not recommended to be treated with antibiotics, EcN might be an alternative medication. However, because a harmless E. coli strain might be converted into a Stx-producer after becoming host to a stx encoding prophage, we tested EcN for stx-phage genome integration. Our experiments revealed the resistance of EcN towards not only stx-phages but also against the lambda phage. This resistance was not based on the lack of or by mutated phage receptors. Rather the expression of certain genes (superinfection exclusion B (sieB) and a phage repressor (pr) gene) of a defective prophage of EcN was involved in the complete resistance of EcN to infection by the stx- and lambda phage. Obviously, EcN cannot be turned into a Stx producer. Furthermore, we observed EcN to inactivate phages and thereby to protect E. coli K-12 strains against infection by stx- as well as lambda-phages. Inactivation of lambda-phages was due to binding of lambda-phages to LamB of EcN whereas inactivation of stx-phages was caused by a thermostable protein of EcN. These properties together with its ability to inhibit Stx production make EcN a good candidate for the prevention of illness caused by EHEC and probably for the treatment of already infected people.
2018-06-14 | GSE109932 | GEO
Project description:Escherichia coli Nissle 1917 (EcN) is a probiotic used for treatment of intestinal disorders. EcN improves gastrointestinal homeostasis and microbiota balance; however little is known about how this probiotic delivers effector molecules to the host. Outer membrane vesicles (OMVs) are constitutively produced by gram-negative bacteria and have a relevant role in bacteria-host interactions. Here we performed proteomic analysis of EcN OMVs. Using 1D SDSD-PAGE and highly sensitive LC-MS/MS analysis we identified 192 EcN vesicular proteins with high confidence in three independent experiments. Of these proteins, 18 were encoded by strain-linked genes and 57 were common to pathogen-derived OMVs. These proteins may contribute to the ability of this probiotic to colonize the human gut as they fulfil functions related to adhesion to host tissues, immune modulation or bacterial survival in host niches. This study describes the first global OMV proteome of a probiotic strain and provides evidence that probiotic-derived OMVs contain proteins that can target these vesicles to the host and mediate their beneficial effects on intestinal function.
2013-12-19 | PXD000367 | Pride
Project description:Bacteria that colonize the human gut must withstand a variety of stressors, including detergent-like compounds known as bile acids. Here, we investigated how bile acids found in the human cecum and colon impact the behavior of the probiotic strain Escherichia coli Nissle 1917 (EcN). We found that lithocholic acid (LCA), which is a microbiota-derived secondary bile acid, promotes the formation of a distinctive surface-coating biofilm by EcN, including on an organoid-derived model of the human colonic epithelium. Mechanistic investigations, including RNA-sequencing, revealed that LCA upregulates the production of several components of flagella, which are essential for LCA-induced biofilm formation and form part of the biofilm extracellular matrix.
2025-05-26 | GSE297446 | GEO
Project description:To determine whether calprotectin can elicit any transcriptional response in the probiotic E. coli Nissle 1917(EcN), EcN was treated with 200 ug/g of calprotectin in log phase.
2023-08-16 | GSE211418 | GEO
Project description:We performed comparative transcriptomic profiling of E. coli Nissle 1917 (EcN) to determine the effect of microgravity (MG) on cell growth and metabolism.
2020-05-21 | GSE147272 | GEO
Project description:Escherichia coli Nissle 1917 (EcN), a well-known Gram-negative probiotic bacterium, has been widely used to treat various intestinal disorders and has gained recognition as a promising platform for diverse biotechnological applications, validated by numerous institutions and researchers over the years However, despite its importance, developing suitable expression systems in EcN has been challenging due to the difficulty in meeting key criteria such as non-toxicity, biocompatibility, and tunable expression. Recently, we identified a gene cluster in EcN responsible for tagatose utilization, challenging the conventional belief that E. coli cannot metabolize tagatose. D-tagatose, a rare, low-calorie sugar naturally found in small amounts in dairy products and fruits, is recognized as safe by the FDA. Due to its poor digestibility in humans, only certain enteric microorganisms are capable of metabolizing it. In this study, we investigated the regulatory elements within this gene cluster using high-throughput differential RNA sequencing (dRNA-seq) and developed a tagatose-inducible expression plasmid, creating a tunable gene expression system for engineering EcN. We evaluated the performance of this novel system and successfully applied it to overproduce a pharmaceutical protein and an industrial enzyme under both aerobic and anaerobic conditions.
2025-02-19 | GSE280463 | GEO
Project description:Escherichia coli Nissle 1917 isolate:EcN Transcriptome or Gene expression
| PRJNA932535 | ENA
Project description:Escherichia coli Nissle 1917 isolate:EcN Transcriptome or Gene expression
| PRJNA1078923 | ENA
Project description:We analyzed the protein composition of the OMVs from E. coli Nissle 1917 (EcN) and the OmpA-depleted EcN strain
2024-12-02 | PXD058507 |