Dataset Information

Bulk RNA-seq analysis of wildtype and MYF5/MYOD/MYF6 knockdown porcine embryo

ABSTRACT: Bulk RNA-seq analysis of wildtype and MYF5/MYOD/MYF6 knockdown porcine embryo

PROVIDER: PRJNA1216616 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32153881_1.fastq.gz	Fastqsanger.gz
	SRR32153881_2.fastq.gz	Fastqsanger.gz
	SRR32153882_1.fastq.gz	Fastqsanger.gz
	SRR32153882_2.fastq.gz	Fastqsanger.gz
	SRR32153883_1.fastq.gz	Fastqsanger.gz

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Project description:The pig is an important animal model increasingly used for biomedical research, particularly in transplantation strategies involving xenotransplantation or the development of human organs in pig for exotransplantation. Pigs, however, are less characterized than other animal models. In this study, we produced wildtype (WT) pig embryos via somatic cell nuclear transfer (SCNT) technology and compared them to skeletal muscle null embryos (lacking MYF5/MYOD/MYF6) at embryonic day 41, 62, and 90, critical stages of porcine myogenesis. Magnetic resonance imaging (MRI) and histological analyses revealed progressive development of skeletal muscle in WT embryos but not in null embryos whereas development of viscera progressed equally in both groups. Molecular analyses highlighted dynamic changes in myogenic gene expression and myofiber formation, demonstrating an organized progression of myogenesis in WT embryos. Morphologically, the null embryos exhibited abnormalities, including marked edema and underdeveloped limbs. MRI revealed severe skeletal abnormalities, including the absence of ribs, sternum, and associated vertebral malformations. In addition, histological analysis confirmed the complete lack of myofiber formation. Immunohistochemical analysis revealed the absence of myogenic stem cells and muscle differentiation, and RNA sequencing demonstrated that the skeletal muscle development process was entirely disrupted in null embryos. Additionally, analysis of neuromuscular junctions (NMJs) in the null embryos revealed that functional NMJ formation was absent, consistent with the lack of skeletal muscle formation. Importantly, these defects culminated in embryonic lethality after day 62 in the null embryos. We determined that the myogenic regulatory gene cascade is crucial for porcine embryo development and viability. The deletion of skeletal muscle is essential for the creating a vacant niche to allow for complementation of null porcine embryos with human induced pluripotent stem cells. Characterization of this skeletal muscle null pig model provide an important platform for engineering humanized muscle in gene-edited pigs.

Dataset Information

Bulk RNA-seq analysis of wildtype and MYF5/MYOD/MYF6 knockdown porcine embryo

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