Project description:This experiment aims on the identification of serine hydrolases from a complex thermophile community that live in a hot vent in Kamchatka Peninsula based on in vivo labelling with FP-alkyne directly in the hot spring and subsequent analysis using metagenomics/metaproteomics. To this end, sediment samples were collected and treated using the following three conditions. DMSO- treated control FP-alkyne labelled Samples for each condition were prepared in triplicate, resulting a total number of 6 samples per spring. Labelling was performed using 4 µM of the probe FP-alkyne and incubation for 2 h in the hot spring.
Project description:This study examined archaeal lipidome of a total of 52 sediment and soil samples across a wide range of environmental gradients, including sediment from hot springs in Tengchong, Yunan Province, sediment from acid mine drainages in Anhui and Guangdong provinces, permafrost soil from Tibet Plateau, soil from Western Sichuan Plateau, surface sediment of cold seeps and sediment core material from the South China Sea, and sediment from the East China Sea.