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SiPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on fibronectin coated glass coverslips

ABSTRACT: siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on fibronectin coated glass coverslips

PROVIDER: PRJNA1217813 | ENA |

REPOSITORIES: ENA

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siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on fibronectin coated glass coverslips

Project description:Mechanosensitive ion channels have emerged as fundamental proteins in sensing extracellular matrix (ECM) mechanics. Among those, Piezo1 has been proposed as a key mechanosensor in cells. However, whether and how Piezo1 senses time-dependent ECM mechanical properties (i.e., viscoelasticity) remains unknown. To address this question, we combined an immortalised mesenchymal stem cell (MSC) line with adjustable Piezo1 expression with soft (400 Pa) and stiff (25 kPa) viscoelastic hydrogels with independently tuneable Young’s modulus and stress relaxation. Here, we propose that Piezo1 is an important sensor of stiffness at this range (25kPa) consistent with the molecular clutch model. By performing RNA sequencing (RNA-seq), we identified the transcriptomic phenotype of MSCs response to matrix viscoelasticity and Piezo1 activity, highlighting gene signatures that drive MSCs mechanobiology at this elasticity scale.

2025-08-20 | GSE288464 | GEO

siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on soft hydrogels

Project description:Mechanosensitive ion channels have emerged as fundamental proteins in sensing extracellular matrix (ECM) mechanics. Among those, Piezo1 has been proposed as a key mechanosensor in cells. However, whether and how Piezo1 senses time-dependent ECM mechanical properties (i.e., viscoelasticity) remains unknown. To address this question, we combined an immortalised mesenchymal stem cell (MSC) line with adjustable Piezo1 expression with soft (0.4 kPa) and stiff (25 kPa) viscoelastic hydrogels with independently tuneable Young’s modulus and stress relaxation. Here, we propose that Piezo1 is an important sensor of stiffness at this range (25kPa) consistent with the molecular clutch model. By performing RNA sequencing (RNA-seq), we identified the transcriptomic phenotype of MSCs response to matrix viscoelasticity and Piezo1 activity, highlighting gene signatures that drive MSCs mechanobiology at this elasticity scale.

2025-08-20 | GSE288465 | GEO

siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on stiff hydrogels

2025-08-20 | GSE288423 | GEO

siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on stiff hydrogels

Project description:siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on stiff hydrogels

| PRJNA1217413 | ENA

siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on soft hydrogels

Project description:siPiezo1 or scRNA immortalised MSC cell line (Y201) cultured on soft hydrogels

| PRJNA1217810 | ENA

Protein expression in primary murine dermal fibroblasts grown on fibronectin vs. plastic

Project description:We were interested in identifying proteins that are regulated in response to the extracellular matrix molecule, fibronectin, in dermal fibroblasts. To determine proteins regulated by exposure to fibronectin, primary murine dermal fibroblasts were cultured on tissue culture plastic or on tissue culture plates coated with a thin layer of fibronectin (2 μg/cm2). Fibroblasts were seeded at 35,000 cells/10 cm2 and were harvested 48 hours later. Cell pellets from these fibroblasts were shipped to Kinexus Bioinformatics Corp. (Vancouver, B.C.) where they were lysed and analyzed using the Kinex TM Antibody Microarray (KAM 1.2), which contains 800 (500 pan-specific and 300 phosphorylation site-specific) antibodies. This enabled us to identify candidate proteins regulated by exposure to fibronectin.

2011-06-22 | GSE30135 | GEO

Effect of high or low chronic shear stress on microvascular endothelial cells cultured on native- versus AGE-fibronectin

Project description:Pulmonary microvascular endothelial cells were cultured on the inner surface of fibers (each 700 µm inner diameter) coated with either with native- or advanced glycation endproduct (AGE) modified fibronectin in hollow-fiber cartridges (FiberCell Systems, Inc.). Duration of shear stress application was 10 days. Shear stress was gradually increased to 17 dyne/cm2 in 2 samples (GSM32266: native fibronectin high shear stress or GSM45608: AGE-fibronectin) or was kept at constant low level of 1 dyne/cm2 in 1 sample (GSM41248: native fibronectin low shear stress). Keywords: parallel sample

2006-01-01 | GSE2424 | GEO

Gene expression analysis of myeloid cells cultured in the presence of fibronectin and/or anti-ILT3 16C5

Project description:RNA sequencing of monocyte-derived dendritic cells, tolerogenic dendritic cells, unpolarized macrophages, and M1, M2a, and M2c macrophages differentiated in vitro on PBS- or fibronectin-coated wells, in the presence of control IgG1 or anti-ILT3 16C5

2024-05-01 | GSE175354 | GEO

Effect of high or low chronic shear stress on microvascular endothelial cells cultured on native- versus AGE-fibronectin

2005-12-31 | E-GEOD-2424 | biostudies-arrayexpress

Differential gene expression between Salmonella Typhimurium biofilms on glass vs cholesterol surfaces

Project description:Salmonella spp. biofilms have been implicated in persistence in the environment and plant surfaces. In addition, Salmonella is able to form biofilms on the surface on cholesterol gallstones. The ability of Salmonella spp. on these surfaces is superior to biofilm formation on surfaces on glass or plastic. Thus, we hypothesized that Salmonella gene expression is specific during biofilm development on cholesterol surfaces. Flow through assays were performed whereby S. Typhimurium was inoculated into chambers coated with glass or cholesterol. At 24h post-inoculation, planktonic (from the flow through), biofilms (from glass or cholesterol) were collected. Thus we had 4 samples: Planktonic (2) and Biofilms (2), each with 2 biological replicates

2013-07-09 | E-GEOD-48604 | biostudies-arrayexpress

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