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Ribo-seq analysis elucidates the dynamic translation landscape of yak ovarian tissue across different reproductive stages

ABSTRACT: Ribo-seq analysis elucidates the dynamic translation landscape of yak ovarian tissue across different reproductive stages

PROVIDER: PRJNA1219079 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32222036_1.fastq.gz	Fastqsanger.gz
	SRR32222036_2.fastq.gz	Fastqsanger.gz
	SRR32222037_1.fastq.gz	Fastqsanger.gz
	SRR32222037_2.fastq.gz	Fastqsanger.gz
	SRR32222038_1.fastq.gz	Fastqsanger.gz

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Ribo-seq analysis elucidates the dynamic translation landscape of yak ovarian tissue across different reproductive stages

Project description:Translation, as a crucial link in the transmission of genetic information, plays an indispensable regulatory role in follicular development, ovulation, corpus luteum formation, and degeneration. In order to deeply analyze the molecular mechanisms of reproductive regulation at the translation level, our study focused on the ovarian tissues of yaks at different reproductive stages to analyze gene expression changes using Ribosome profiling and integrating RNA-seq data. Meanwhile, the small open reading frames (sORFs) of ovarian tissue was characterized, and the impact of the translation efficiency of focused genes on their sequence feature was analyzed. The results showed that over 80% of genes in the two groups exhibited inconsistent changes at the transcription and translation levels, indicated that the changes in genes at both levels were complex and not simply synergistic. Upon the pathway enrichment analysis, the signaling pathways involved in these differentially expressed genes were further clarified, such as the estrogen signaling pathway PI3K-Akt, MAPK, calcium signaling pathway and ovarian steroidogenesis. Further research has found that some genes related to ovarian function have inconsistent expression at two levels and exhibit dynamic changes in translation activity, including PALB2, EGR2, BMP7, SOX9, PIK3R2, and WNT2B. Besides, we identified 66 predicted translatable small open reading frames (sORFs) and also explored the impact of upstream ORFs (uORFs) on the translation efficiency of downstream major ORFs (mORFs). This study systematically revealed the gene expression characteristics of ovarian tissues in yaks at different reproductive stages from the perspective of translational level for the first time, providing a new perspective for a deeper understanding of the physiological mechanisms of yak reproduction.

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Ribo-seq reveals translational landscapes during yak testicular development process and cattle-yak.

Project description:This study used yak and cattle-yak testes from different developmental stages as materials to construct a complete translation map of the testes, and integrated transcriptome and translation results to explore gene expression changes during the sexual maturation process of yak testes. This study utilized Ribo seq technology to construct a transcriptome map of yak testicular development, revealing that the expression of genes related to spermatogenesis is specifically translated and regulated at different developmental stages. In addition, many unknown open reading frames (ORFs) in the testes have been newly identified.

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10x genomics single cell sequencing of yak testis at different developmental stages

Project description:Testis is the most important reproductive organ of men, and the integrity of its physiological function is crucial for the successful production of sperm. In this study, single-cell RNA sequencing technology was used to construct cell expression profiles of yak at different developmental stages and adult cattle-yak testicular tissues. The identification results of cell subsets and marker genes were analyzed, and the possible mechanisms were predicted.

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The transcriptome sequencing of yak (Bos grunniens)

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Ribo-seq reveals translational landscapes during yak testicular development process and cattle-yak.

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Dynamic changes in cellular atlases and communication patterns within yak ovaries across diverse reproductive states unveiled by single-cell analysis [scRNAseq_Yak_Ovary_Pregnancy_2]

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Dynamic changes in cellular atlases and communication patterns within yak ovaries across diverse reproductive states unveiled by single-cell analysis [scRNAseq_Yak_Ovary_Anestrus_1]

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Dynamic changes in cellular atlases and communication patterns within yak ovaries across diverse reproductive states unveiled by single-cell analysis [scRNAseq_Yak_Ovary_Pregnancy_1]

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