Project description:The complete mitochondrial genome of the strawberry aphid Chaetosiphon fragaefolii Cockerell, 1901 (Hemiptera: Aphididae) from California, USA
Project description:The aphid Chaetosiphon fragaefolii Cockerell, 1901 is an agricultural pest and known vector of strawberry viruses. To better understand its biology and systematics, we performed a genomic analysis on C. fragaefolii collected from Quinalt strawberry plants from Pacific Grove, Monterey county, California, USA using Oxford Nanopore and Illumina sequencing. The resulting data were used to assemble the aphids complete mitogenome. The mitogenome of C. fragaefolii is 16,108 bp in length and contains 2 rRNA, 13 protein-coding, and 22 tRNA genes (GenBank accession number LC590896). The mitogenome is similar in content and organization to other Aphididae. Phylogenetic analysis of the C. fragaefolii mitogenome resolved it in a fully supported clade in the tribe Macrosiphini. Analysis of the cox1 barcode sequence of C. fragaefolii from California found exact and nearly identical sequences to C. fragaefolii and Chaetosiphon thomasi Hille Ris Lambers, 1953, suggesting the two species are conspecific.
Project description:Venom injected at oviposition is crucial for successful reproduction in most parasitoid wasp species (Moreau & Asgari 2015; Poirié et al. 2014). The venom of the pea aphid parasitoid Aphidius ervi was analyzed previously using a combined transcriptomic and proteomic approach (Colinet et al. 2014) and we applied similar methods here to compare the venom composition in Lysiphlebus fabarum, that also used the pea aphid as host. Venom was extracted from the L. fabarum venom gland and analyzed using 1D gel electrophoresis and mass spectrometry. A total of 35 L. fabarum proteins were identified as putative venom proteins using these results combined with available transcriptomic data (Dennis et al. 2017) and the genomic data.