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DIFFERENTIAL TEMPERATURE-INDUCED RESPONSES IN ORAL AND SKIN KERATINOCYTES

ABSTRACT: DIFFERENTIAL TEMPERATURE-INDUCED RESPONSES IN ORAL AND SKIN KERATINOCYTES

PROVIDER: PRJNA1221942 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32325134_1.fastq.gz	Fastqsanger.gz
	SRR32325134_2.fastq.gz	Fastqsanger.gz
	SRR32325135_1.fastq.gz	Fastqsanger.gz
	SRR32325135_2.fastq.gz	Fastqsanger.gz
	SRR32325136_1.fastq.gz	Fastqsanger.gz

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DIFFERENTIAL TEMPERATURE-INDUCED RESPONSES IN ORAL AND SKIN KERATINOCYTES

Project description:The epidermis of skin and oral mucosa is constantly exposed to various environmental stimuli, including temperature changes. In particularly extreme conditions, such as excess heat or cold, significant injury may occur. Oral and skin keratinocytes exhibit tissue-specific differences in wound healing outcomes and the transcriptomic response to injury. This study investigated if skin and oral keratinocytes also have differential responses to heat and cold-induced injury. Oral keratinocytes (TIGK) were found to exhibit an enhanced viability following heat-induced injury compared to skin keratinocytes (HaCaT). However, there were no discernible differences between skin and oral keratinocyte viability following cold-induced injury. To examine the transcriptomic differences between skin and oral keratinocytes in response to temperature-induced injury, we generated an mRNA-sequencing gene expression dataset. Differentially expressed genes (DEGs) including heat shock proteins (HSPs) were identified between HaCaT and TIGK at baseline (37°C) and after heat (60°C) or cold-induced (-25°C) injury. Our comparative analyses suggest that skin and oral keratinocytes exhibit transcriptomic differences at baseline and in their responses to heat or cold exposure. The enhanced heat tolerance of TIGK relative to HaCaT may be due to an advantageous expression of a subset of HSPs at baseline in TIGK. Our work also provides a source of skin and oral keratinocyte gene expression data following heat and cold-induced injury that can be used for future analyses.

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Intrinsic differences between oral and skin keratinocytes

Project description:The morphology and the behavior of skin and oral tissue keratinocytes are different. One significant dissimilarity between the two sites is the response to injury. Oral and skin keratinocytes have intrinsic differences in the response to injury and such differences are reflected in gene expression profiles. We used microarrays to investigate differences in global gene expression patterns between baseline skin and oral epithelium sheets without their underlying connective tissue. Paired skin and oral epithelium was separated from the dermis for RNA extraction and hybridization on Affymetrix microarrays. Skin epidermal tissues were obtained from the tail of mice and oral epidermal tissues were obtained from the hard palate. Enzymatically isolated epithelium was used for analysis.

2014-06-15 | E-GEOD-56135 | biostudies-arrayexpress

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Transcriptomic Differences between Oral and Skin Keratinocytes

Project description:Compared to skin wounds, oral mucosal wounds heal more rapidly with less inflammation, faster re-epithelialization, and minimal scarring. One cell type that may be a differentiating factor is keratinocytes. Using immortalized skin and oral keratinocytes, HaCaT and TIGK, respectively, we found that oral keratinocytes have an enhanced migratory and proliferative capacity. To examine the transcriptomic differences that underlie the improved healing abilities of oral keratinocytes, we generated an RNA-sequencing (RNA-seq) gene expression dataset utilizing HaCaT and TIGK. Differentially expressed genes (DEGs) were identified between HaCaT and TIGK at baseline and throughout the injury response. Gene Ontology (GO) or Reactome enrichment analysis was performed to understand the biological significance of DEGs. Processes related to inflammation and migration were uniquely enriched in TIGK. Additionally, TIGK retained its enhanced migratory capacity when grown on various basement membrane (BM) and extracellular matrix (ECM) substrates. This may be due to the differential expression of matrix metalloproteinases (MMPs) and integrins between HaCaT and TIGK. We also identified genes differentially over or under-expressed in HaCaT and TIGK following injury when compared to each respective cell type’s unwounded gene expression levels. Lastly, we showed that the post-injury secretome of TIGK promotes keratinocyte migration. Our comparative analyses suggest specific transcriptomic differences between oral and skin keratinocytes at unwounded baseline and in response to injury may underlie the distinct wound healing phenotypes seen in these two tissues. This work also provides a source of HaCaT and TIGK gene expression data over healing that can be used for future analyses.

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Transcriptomic Differences between Oral and Skin Keratinocytes

Project description:Transcriptomic Differences between Oral and Skin Keratinocytes

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MicroRNA differential expression in skin and oral mucosal epithelium

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