Project description:The extracellular matrix (ECM) is a complex network comprising hundreds of proteins, serving not only a structural role in shaping multicellular organisms but also participating in signaling processes. Moreover, the ECM exhibits high tissue specificity and undergoes significant alterations with aging progression. We analyzed the proteome of ECM produced by liver aging-specific fibrotic niche cells in old mice to obtain an age-related proteome profile. Our analysis revealed the identification of 714 quantifiable proteins, with 126 classified as matrisome proteins according to the matrisome database. Notably, proteins significantly upregulated in the ECM of liver aging-specific fibrotic niche cells indicated characteristics reminiscent of ECM formation (Eln, Mfap4, Lox, Tgfb2, Thbs2) and immune cell recruitment (Cxcl12), akin to those originating from fibrotic regions. Our findings suggest that this study may contribute to elucidating heightened fibrotic characteristics associated with the aging process.
Project description:Age-dependent fibrosis accumulated in the liver includes driver cell types of aging. We used single cell RNA sequencing (scRNA-seq) from specially digested liver to analyze the age-related fibrosis.
Project description:Age-dependent fibrosis accumulated in the liver includes driver cell types of aging. We used single cell RNA sequencing (scRNA-seq) from specially digested liver to analyze the age-related fibrosis.
Project description:Age-dependent fibrosis accumulated in the liver includes driver cell types of aging. We used single cell RNA sequencing (scRNA-seq) from specially digested liver to analyze the age-related fibrosis.