Similar Datasets
Project description:Soil Aquifer Treatment (SAT) is recognized as a cost-effective approach to reduce contaminants of emerging concern (CECs) from Wastewater Treatment Plant (WWTP) effluents. However, its efficiency in removing the associated biological effects is still poorly understood. Here, we evaluated the efficiency of three pilot SAT systems, two of them enhanced with reactive barriers containing different proportions of sand and organic materials, in removing toxicity associated to CECs. SATs were fed with secondary effluents from the Palamós WWTP (N.E. Spain) during two consecutive campaigns scheduled before and after the summer of 2020. Fifteen water samples were collected from the WWTP effluent, below the barriers and 15 m into the aquifer. Transcriptomic analyses of zebrafish embryos exposed to the corresponding water extracts revealed a wide range of toxic activities in the WWTP effluents. Results demonstrated that the associated responses were reduced by more than 70% by SAT, achieving control levels in some cases. Similar results were obtained when human HepG2 hepatic cells were tested for cytotoxic and dioxin-like responses. Toxicity reduction appeared to be partially determined by the reactive barrier composition and/or SAT managing and was correlated with the removal of CECs by SAT. In conclusion, SAT appears to be a very promising approach for efficiently reducing the effects of recalcitrant pollutants from WWTP secondary effluents on the environment and human health.
2024-11-01 | GSE240250 | GEO
Project description:In this study, adult male rainbow darter were collected from three sites in the Grand River in the fall season for four consecutive years (2011-2014), from 3 sites: a reference site (US2) located in the non-urbanized area, and two exposure sites (DSW and DSK1) located dowstrem of two secondary wastewater treatment palnts (WWTP) (Waterloo and Kitchener). Kitchener WWTP has been undergone infrastructure upgrades that were initiated in the summer of 2012. The goal of this project was to see whether the implemented upgrades can be reflected in the liver transcriptome response indicative of recovery.The RNA from male rainbow darter was exctracted from liver tissue and used for further gene expression analysis.
2017-02-21 | GSE95083 | GEO
Project description:Pre B cell leukemia homeobox 1 (Pbx1) regulates the balance between self-renewal and differentiation of hematopoietic stem cells, and maintains proto-oncogenic transcriptional pathways implicated in several tumors. Its aberrant expression was found in a subset of myeloproliferative neoplasms (MPN) patients bearing the JAK2V617F mutation. To investigate if Pbx1 contributes to MPN, and to explore its potential as therapeutic target, we generated a new mouse strain, that we called JP, by crossing a known JAK2V617F inducible knock-in MPN model with a Pbx1 conditional-ko. In JP mice we can simultaneously activate the human JAK2 mutation and delete Pbx1. Typical MPN features, such as thrombocytemia and granulocytosis, did not develop in the absence of Pbx1. Erythrocytosis, initially displayed by JP mice, gradually resolved over time. Moreover, splenic myeloid metaplasia and in vitro cytokine independent growth were rescued by Pbx1 inactivation. Through RNA-Sequencing we analyzed molecular pathways downstream of Pbx1 and involved in MPN maintenance in stem and progenitor cells. The aberrant transcriptome in the MPN model compared to wild-type was rescued by the absence of Pbx1. Our results demonstrate that inhibition of the Pbx1-driven transcriptional program is beneficial in MPN. Modulation of Pbx1 activity by direct targeting or by targeting its downstream mediators might thus represent a novel therapeutic approach.
2021-10-21 | GSE153482 | GEO
Project description:Interventions: No Group:No intervention
Primary outcome(s): CRE screening results
Study Design: Sequential
| 2699279 | ecrin-mdr-crc
Project description:The physiological adaptations of the WWTP isolate T. elongata str. LP2 during an oxic/anoxic shift experiment were analyzed by HRMS-proteomics. E. coli was used as reference organism as it shares several metabolic capabilities and is regularly introduced to wastewater treatment plants, but without succeeding there. In contrast to E. coli, T. elongata was able to steadily grow and did not need to activate additional metabolic pathways for anaerobic energy harvest. At the same time, given substrates were exploited far more efficient than by E. coli. Based on the acquired data, we hypothesize that T. elongata shows high metabolic robustness when encountering the WWTP which enables continuous growth to successfully compete in this environment.
2019-03-18 | PXD005211 | Pride
Project description:ESBL and CRE isolates from raw dog food specimens
| PRJNA733494 | ENA