Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1226404 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Enrichment of pluripotent stem cell-derived hepatocyte-like cells
| PRJNA341955 | ENA
Project description:Derivation of a CDX2+/p63+ human cytotrophoblast stem cell from pluripotent stem cells in defined conditions
| PRJNA274618 | ENA
Project description:Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
| PRJNA102719 | ENA
Project description:Transcriptomic profiling of human pluripotent stem cell derived ROR1+ cells
| PRJNA369261 | ENA
Project description:Gene expression data from human induced pluripotent stem cells, induced pluripotent stem cell-derived human neural stem/progenitor cells, and iPSC-derived cerebral cortical neurons
| PRJNA260838 | ENA
Project description:Integrative epigenomic and transcriptomic characterization of hepatocyte-like cells differentiated in vitro from human induced pluripotent stem cells in comparison with primary human hepatocytes. This study comprises single cell RNA-seq, bulk mRNA-seq, ATAC-seq and RRBS.
| EGAS00001004201 | EGA
Project description:Modeling CMT1A with human induced pluripotent stem cell derived Schwann cells
| PRJNA287351 | ENA
Project description:Human pluripotent stem cell-derived mesenchymal stem cells regenerate injured GUNN rat liver
| PRJNA382677 | ENA
Project description:Human intestinal tissue with adult stem cell properties derived from pluripotent stem cells
| PRJNA245172 | ENA
Project description:Signal differences between TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi in representative cardiac and hepatic gene expression in mRNA microarray analysis. INTRODUCTION: Non-genetic purification methods for pluripotent stem cell-derived hepatocyte-like cells are useful for liver regenerative therapy and pharmaceutical applications. METHODS: Fluorescent activated cell sorting (FACS) was used to separate cells by combining two parameters: cellular mitochondrial content evaluated by the mitochondrial membrane potential-dependent fluorescent probe (TMRM) and immunocytochemical detection of activated leukocyte cell adhesion molecule (ALCAM). This method was applied to murine fetal, human embryonic stem cell (ESC)-derived, and human induced pluripotent stem cell (iPSC)-derived cell-mixtures. Separately sorted cell fractions were evaluated by quantitative PCR, immunohistochemistry, and cytochemistry for HNF4a, AFP, and albumin mRNA and/or protein expression. RESULTS: Hepatocyte-like cells were highly segregated into the high TMRM signal and ALCAM-positive population. The purity of hepatocyte-like cells derived from human iPSCs was 97 ± 0.38% (n = 5). CONCLUSIONS: This hepatocyte-like cell purification method may be applicable to quality control of cells for liver regenerative cell therapy and pharmaceutical development.
2020-03-01 | GSE126812 | GEO