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Rossomyrmex anatolicus

ABSTRACT: Rossomyrmex anatolicus

PROVIDER: PRJNA1228050 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR33206785_1.fastq.gz	Fastqsanger.gz
	SRR33206785_2.fastq.gz	Fastqsanger.gz

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Effect of Rubus anatolicus Leaf Extract on Glucose Metabolism in HepG2, CRI-D2 and C2C12 Cell Lines.

Project description:Introduction:The aim of this study was to assess the effects of Rubus anatolicus on glucose metabolism in HepG2, CRI-D2 and C2C12 cell lines. Materials and Methods:R. anatolicus was collected in Golestan province, Iran. Three different cell lines HepG2 (human liver cell), CRI-D2 (mice pancreatic cell) and C2C12 (rat myoblast) were used for cell culture experiments. Cell viability was measured using MTT assay. Cells were treated with various concentrations of the extract (6.25-400 ?g/mL) and then the extracellular glucose level and intracellular glycogen content were measured using colorimetric methods. The insulin level of the culture medium was measured using the ELISA method. Results:Our findings showed that R. anatolicus extract enhances glucose uptake and consumption by all three cell lines. The R. anatolicus extract exposure also elevated cellular glycogen content in HepG2 and C2C12 cells (for 200 and 100 ?g/mL) significantly. We found a significant increase in glucose uptake and consequently higher stimulation of insulin secretion in CRI-D2 cell pancreatic cells treated with R. anatolicus extract. Conclusion:The R. anatolicus appears to activate glucose uptake and cellular glycogen synthesis probably by activating the glycogenesis or inhibition of glycogenolysis pathways. The extract enhances insulin secretion in the pancreatic cells by increased glucose uptake.

| S-EPMC7166088 | biostudies-literature

Hop1

Project description:Pest status and prevalence of Hoplopteridius lutosus anatolicus Osella & Lodos (Col.: Curculionidae) in Saffron and DNA Barcoding Studies

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Whole genome bisulfite sequencing of liver samples of five mammals (human, rhesus macaque, mouse, rat and dog)

Project description:This study aims to investigate the DNA methylation patterns at transcription factor binding regions and their evolutionary conservation with respect to binding activity divergence. We combined newly generated bisulfite-sequencing experiments in livers of five mammals (human, macaque, mouse, rat and dog) and matched publicly available ChIP-sequencing data for five transcription factors (CEBPA, HNF4a, CTCF, ONECUT1 and FOXA1). To study the chromatin contexts of TF binding subjected to distinct evolutionary pressures, we integrated publicly available active promoter, active enhancer and primed enhancer calls determined by profiling genome wide patterns of H3K27ac, H3K4me3 and H3K4me1.

2022-10-20 | E-MTAB-11946 | biostudies-arrayexpress

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