Project description:Superoxide dismutase (EC 1.15.1.1) (SOD) is a standout amongst the most critical metal containing enzymes that can act as a main line of defense against oxidative stress. There has always been an increased interest globally to identify new plant sources with high SOD activity that are pharmacologically potent and have minimum side effects for their use as preventive medicine and in food industry. Trachyspermum ammi seeds showed high SOD activity and the enzyme was purified to homogeneity using various downstream processing techniques like; Salt precipitation, dialysis, IEX, Gel permeation, NATIVE- & SDS-PAGE.
Project description:Superoxide dismutase (EC 1.15.1.1) (SOD) is a standout amongst the most critical metal containing enzymes that can act as a main line of defense against oxidative stress. There has always been an increased interest globally to identify new plant sources with high SOD activity that are pharmacologically potent and have minimum side effects for their use as preventive medicine and in food industry. Trachyspermum ammi showed high SOD activity and the enzyme was purified to homogeneity using various downstream processing techniques like; Salt precipitation, dialysis, IEX, Gel permeation, NATIVE- and SDS-PAGE. Biochemical characterization with respect to optimum operational parameters viz., pH, Temperature, Stability, Concentration etc., was done. Kinetic studies for determination of Km, Vmax, Temperature coefficient, Activation energy have also been accomplished.
Project description:This study aims to investigate the DNA methylation patterns at transcription factor binding regions and their evolutionary conservation with respect to binding activity divergence. We combined newly generated bisulfite-sequencing experiments in livers of five mammals (human, macaque, mouse, rat and dog) and matched publicly available ChIP-sequencing data for five transcription factors (CEBPA, HNF4a, CTCF, ONECUT1 and FOXA1). To study the chromatin contexts of TF binding subjected to distinct evolutionary pressures, we integrated publicly available active promoter, active enhancer and primed enhancer calls determined by profiling genome wide patterns of H3K27ac, H3K4me3 and H3K4me1.
Project description:Whole genome sequencing of the Arabidopsis thaliana dot5-1 transposon insertion line described in Petricka et al 2008 The Plant Journal 56(2): 251-263.