Project description:Retrospective analysis of RNAi hits derived from Wang et. al. has found that genes with greater transcripts-per-million (TPM) in S. mansoni are more likely to produce physical phenotypes. To create the best rate-of-return in our large-scale RNAi screen to identify essential schistosome genes that produce physical phenotypes, we quantified TPM of each gene expressed in male parasites after culturing them with female parasites for 72 hours.
Project description:We distrupted the expression of the Schistosoma mansoni zfp-1-1 gene using RNA interference and examined the transcriptional effects by illumina sequencing
Project description:Transcriptional profiling using two subsequent developmental stages of Schistosoma mansoni (Egg vs. Miracidium; Cercaria vs. 7-days-old Schistosomulum; 7-days-old Schistosomulum vs. Adult worms