Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1235322 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Bacterial motility shows a strong evolvable feature depending on the environment. Hyper-motile E. coli could be isolated by evolving non-motile E. coli due to the mutations that enhanced transcriptional expression of the master regulator of the flagellum biosynthesis, FlhDC. These hyper-motile isolates showed reduced growth fitness but with the molecular mechanisms unrevealed. Here we obtained a novel type of hyper-motile isolates by evolving a weakly-motile E. coli K12 strain on the soft agar plates. These isolates carried high accumulated FlhDC proteins and they shared one single point mutation of ClpXV78F. The V78F affected the ATP binding to ClpX via steric repulsive effect and the mutated ClpXP protease lost most of its ability to degraded FlhDC and some other of its known targets. The signal tag of FlhDC for ClpXP recognition was also characterized. Intriguingly, in the hyper-motile strains, the highly enhanced expression of the motility genes was accompanied by the reduced expression of stress resistance genes relating to the reduced fitness of these isolates. Hence, ClpX appeared to be a novel and hot locus during the evolution of bacterial motility and the molecular mechanism of the trade-off between motility and growth was proposed for the first time.
2021-11-15 | GSE165151 | GEO
Project description:Oligonucleotide DNA microarrays were used as a platform to compare C. jejuni isolates from feedlot cattle and human clinical cases from Alberta. Comparative genomic hybridization (CGH) analysis was performed on 87 isolates (46 bovine, 41 human) obtained within the same geographical regions and time frame. In addition, We also performed gene association analysis to determine if any genes may be differentially distributed between human and cattle sources or between clusters dominated by either human or cattle isolates (“human enriched” vs “cattle enriched”). Keywords: Comparative Genomic Hybridization; Genomic epidemiology; Gene-association study
2008-10-17 | GSE13228 | GEO
Project description:ra08-02_clavata-ralstonia - clavata-ralstonia - Clavata1 (Clv1.12) and clavata2 (RPL10) mutants display an increased resistance to the bacteria Ralstonia solanacearum. Transcriptome analysis aims to understand resistance mechanisms. - 3 biological repets Keywords: wt vs mutant comparison
2009-10-30 | GSE18757 | GEO
Project description:rs08-04_wat1-ralstonia - ralstonia infection - identification of the role of the plant cell wall in the interactions between plants and pathogenic agents - Comparison between the mutant and the wt at different time after infection with ralstonia bacteria Keywords: treated vs untreated comparison
2010-08-01 | GSE19081 | GEO
Project description:Bacterial motility shows a strong evolvable feature depending on the environment. Hyper-motile E. coli could be isolated by evolving non-motile E. coli due to the mutations that enhanced transcriptional expression of the master regulator of the flagellum biosynthesis, FlhDC. These hyper-motile isolates showed reduced growth fitness but with the molecular mechanisms unrevealed. Here we obtained a novel type of hyper-motile isolates by evolving a weakly-motile E. coli K12 strain on the soft agar plates. These isolates carried high accumulated FlhDC proteins and they shared one single point mutation of ClpXV78F. The V78F affected the ATP binding to ClpX via steric repulsive effect and the mutated ClpXP protease lost most of its ability to degraded FlhDC and some other of its known targets. The signal tag of FlhDC for ClpXP recognition was also characterized. Intriguingly, in the hyper-motile strains, the highly enhanced expression of the motility genes was accompanied by the reduced expression of stress resistance genes relating to the reduced fitness of these isolates. Hence, ClpX appeared to be a novel and hot locus during the evolution of bacterial motility and the molecular mechanism of the trade-off between motility and growth was proposed for the first time.
2021-11-25 | PXD023645 | Pride
Project description:ra08-02_clavata-ralstonia - clavata-ralstonia - Clavata1 (Clv1.12) and clavata2 (RPL10) mutants display an increased resistance to the bacteria Ralstonia solanacearum. Transcriptome analysis aims to understand resistance mechanisms. - 3 biological repets Keywords: wt vs mutant comparison 6 dye-swap - CATMA arrays
2009-10-30 | E-GEOD-18757 | biostudies-arrayexpress
Project description:rs08-04_wat1-ralstonia - ralstonia infection - identification of the role of the plant cell wall in the interactions between plants and pathogenic agents - Comparison between the mutant and the wt at different time after infection with ralstonia bacteria Keywords: treated vs untreated comparison 20 dye-swap - CATMA arrays
2010-07-31 | E-GEOD-19081 | biostudies-arrayexpress
Project description:Pseudomonas aeruginosa is a versatile opportunistic pathogen, well-known for its adaptability to the challenging conditions of infection in the human host. In chronic biofilm-associated infections, variations in motility and LPS structure are commonly observed adaptive traits. Here, we identified the expression of the O-antigen biosynthetic cluster as a key driver of motility variation among a large number of clinical PA14 clade isolates. Although flagellar presence and function remain intact, increased hydrophobicity in O-specific antigen (OSA)-deficient mutants enhances surface attachment, which limits surface motility but promotes biofilm formation. The modified lipopolysaccharide structure with a lack of OSA also results in increased serum sensitivity, reduced phagocytic uptake, increased aminoglycoside resistance, and enhanced expression of the type III secretion system. These findings suggest that the commonly observed loss of OSA in P. aeruginosa isolates recovered from chronically infected cystic fibrosis lungs may be selectively advantageous. Importantly, we demonstrate that clinically relevant phenotypes, such as motility and antibiotic resistance, are conditional and may not be detectable under all assay conditions. Similarly, the role of OSA expression in bacterial pathogenicity is conditional, with neither its presence nor absence uniformly favoring infection. Instead, dynamic expression—with high levels in the early stages of infection and reduced levels in later stages—appears to be crucial for the establishment and persistence of infection.
2026-09-21 | GSE284794 | GEO
Project description:These series of experiments compares the expression profile of the motility variants of E.coli MG1655 ( Motile and NonMotile isolates) to an isogenic E.coli MG1655 strain in which the IS5 upstream of flhDC has been deleted. The expression profiles of genes in the E.coli MG1655 motile isolate and E.coli MG1655 Non_Motile isolate also compared. Keywords: parallel sample
2005-06-02 | GSE2694 | GEO
Project description:These series of experiments compares the expression profile of the motility variants of E.coli MG1655 ( Motile and NonMotile isolates) to an isogenic E.coli MG1655 strain in which the IS5 upstream of flhDC has been deleted. The expression profiles of genes in the E.coli MG1655 motile isolate and E.coli MG1655 Non_Motile isolate also compared.
2010-06-09 | E-GEOD-2694 | biostudies-arrayexpress